Tourancheau A B, Villalobo E, Tsao N, Torres A, Pearlman R E
Laboratoire de Biologie Cellulaire 4 (URA CNRS 2227), Université Paris-Sud, Bâtiment 444, Orsay Cedex, 91405, France.
Mol Phylogenet Evol. 1998 Dec;10(3):299-309. doi: 10.1006/mpev.1998.0534.
We have reexamined the phylogeny of the ciliates using alpha-tubulin and phosphoglycerate kinase gene sequences. For alpha-tubulin, we have compared the amino acid and nucleotide sequences of 20 species representing seven of the nine classes of the phylum (Karyorelictea, Heterotrichea, Hypotrichea, Oligohymenophorea, Colpodea, Nassophorea, and Litostomatea). The phylogenetic tree resembles a bush from which three monophyletic lineages can be distinguished which correspond to the three classes Hypotrichea, Oligohymenophorea, and Litostomatea. For phosphoglycerate kinase, we have compared the amino acid sequences from 7 species representing three classes (Heterotrichea, Hypotrichea, and Oligohymenophorea). The branching pattern is resolved in three deeply separated branches with an early emergence of the heterotrich. Our comparative analysis shows that if alpha-tubulin phylogeny is not informative at the interclass level, the preliminary data from the phosphoglycerate kinase molecule appear more promising. Nevertheless, at low taxonomic level and at the class level, the resolved phylogenetic relationships inferred from both protein and rRNA sequence data are congruent.
我们利用α-微管蛋白和磷酸甘油酸激酶基因序列重新审视了纤毛虫的系统发育。对于α-微管蛋白，我们比较了代表该门九个纲中的七个纲（核残迹纲、异毛纲、下毛纲、寡膜纲、肾形虫纲、鼻口纲和腹毛纲）的20个物种的氨基酸和核苷酸序列。系统发育树类似于一棵灌木，从中可以区分出三个单系谱系，它们对应于下毛纲、寡膜纲和腹毛纲这三个纲。对于磷酸甘油酸激酶，我们比较了代表三个纲（异毛纲、下毛纲和寡膜纲）的7个物种的氨基酸序列。分支模式在三个深度分离的分支中得到解析，异毛类出现较早。我们的比较分析表明，如果α-微管蛋白的系统发育在纲间水平上没有信息价值，那么来自磷酸甘油酸激酶分子的初步数据似乎更有前景。然而，在低分类水平和纲水平上，从蛋白质和rRNA序列数据推断出的已解析系统发育关系是一致的。
