Hendrix R W, Smith M C, Burns R N, Ford M E, Hatfull G F
Pittsburgh Bacteriophage Institute, Department of Biological Sciences, University of Pittsburgh, Pittsburgh, PA 15260, USA.
Proc Natl Acad Sci U S A. 1999 Mar 2;96(5):2192-7. doi: 10.1073/pnas.96.5.2192.
We report DNA and predicted protein sequence similarities, implying homology, among genes of double-stranded DNA (dsDNA) bacteriophages and prophages spanning a broad phylogenetic range of host bacteria. The sequence matches reported here establish genetic connections, not always direct, among the lambdoid phages of Escherichia coli, phage phiC31 of Streptomyces, phages of Mycobacterium, a previously unrecognized cryptic prophage, phiflu, in the Haemophilus influenzae genome, and two small prophage-like elements, phiRv1 and phiRv2, in the genome of Mycobacterium tuberculosis. The results imply that these phage genes, and very possibly all of the dsDNA tailed phages, share common ancestry. We propose a model for the genetic structure and dynamics of the global phage population in which all dsDNA phage genomes are mosaics with access, by horizontal exchange, to a large common genetic pool but in which access to the gene pool is not uniform for all phage.
我们报告了双链DNA（dsDNA）噬菌体和原噬菌体基因之间的DNA及预测蛋白质序列相似性，这意味着在广泛系统发育范围的宿主细菌中存在同源性。此处报告的序列匹配在大肠杆菌的λ样噬菌体、链霉菌的噬菌体phiC31、分枝杆菌的噬菌体、流感嗜血杆菌基因组中一个先前未被识别的隐蔽原噬菌体phiflu以及结核分枝杆菌基因组中的两个小的类原噬菌体元件phiRv1和phiRv2之间建立了遗传联系，这些联系并非总是直接的。结果表明这些噬菌体基因，很可能所有的dsDNA尾噬菌体，都有共同的祖先。我们提出了一个全球噬菌体群体遗传结构和动态的模型，其中所有dsDNA噬菌体基因组都是镶嵌体，通过水平交换可获取一个庞大的共同基因库，但并非所有噬菌体对该基因库的获取都是均等的。
