Kotha A, Raman R C, Ponrathnam S, Kumar K K, Shewale J G
Chemical Engineering Division, National Chemical Laboratory, India.
Appl Biochem Biotechnol. 1998 Sep;74(3):191-203. doi: 10.1007/BF02825965.
Various glycidyl methacrylate (GMA) copolymers were synthesized by suspension polymerization, using pentaerythritol triacrylate (PETA), trimethylolpropane triacrylate (TMPTA), and trimethylolpropane trimethacrylate (TRIM) as crosslinking comonomers. These copolymers were evaluated for the immobilization of penicillin G acylase. Broad pore-size distribution that was observed was in the range 5-300 nm. Both surface area and pore volume increased with increase in the mole fraction of crosslinking comonomer (increasing crosslink density). The pore volume of the copolymers was more than doubled by including lauryl alcohol as porogen. Binding of penicillin G acylase (PGA) was quantitative on highly crosslinked copolymers. The expression of bound PGA was better on the relatively more hydrophilic GMA-TMPTA and GMA-PETA copolymer supports compared to the GMA-TRIM copolymers. Among the different copolymers studied, GMA-TMPTA copolymer 7411 exhibited highest activity of immobilized penicillin G acylase (167.4 IU/g) with 35.1% expression.
采用悬浮聚合法，以季戊四醇三丙烯酸酯（PETA）、三羟甲基丙烷三丙烯酸酯（TMPTA）和三羟甲基丙烷三甲基丙烯酸酯（TRIM）作为交联共聚单体，合成了多种甲基丙烯酸缩水甘油酯（GMA）共聚物。对这些共聚物进行了青霉素G酰化酶固定化的评估。观察到的宽孔径分布范围为5 - 300 nm。随着交联共聚单体摩尔分数的增加（交联密度增加），表面积和孔体积均增大。通过加入月桂醇作为致孔剂，共聚物的孔体积增加了一倍多。青霉素G酰化酶（PGA）在高度交联的共聚物上的结合是定量的。与GMA - TRIM共聚物相比，在相对更亲水的GMA - TMPTA和GMA - PETA共聚物载体上，结合的PGA表达更好。在所研究的不同共聚物中，GMA - TMPTA共聚物7411表现出最高的固定化青霉素G酰化酶活性（167.4 IU/g），表达率为35.1%。
