Ranasinghe J G, Sakakibara Y, Harada M, Nishiyama K, Liu M C, Suiko M
Department of Biological Resource Sciences, Miyazaki University, Japan.
Biosci Biotechnol Biochem. 1999 Jan;63(1):229-31. doi: 10.1271/bbb.63.229.
A reliable HPLC method was used for the identification of positional isomerism and stereoisomerism of sulfated tyrosine residues in human urine. Upon separation of human urine by ion-pair HPLC on a reverse-phase column, p-tyrosine-O-sulfate (p-TyrS) was identified. Differentiation of the L and D forms was done by using a column with a chiral stationary phase. It was concluded that L-p-tyrosine (L-p-Tyr) which is the predominant tyrosine isomer in the human body, was sulfated and excreted in human urine as a normal constituent. The sulfated forms of D-p-Tyr and m-Tyr could not be detected under these analytical conditions.
采用可靠的高效液相色谱法（HPLC）鉴定人尿中硫酸化酪氨酸残基的位置异构体和立体异构体。通过在反相柱上进行离子对HPLC分离人尿，鉴定出对-酪氨酸-O-硫酸盐（p-TyrS）。使用具有手性固定相的色谱柱对L型和D型进行区分。得出的结论是，L-对-酪氨酸（L-p-Tyr）作为人体中主要的酪氨酸异构体，被硫酸化并作为正常成分排泄于人尿中。在这些分析条件下，未检测到D-p-Tyr和间-Tyr的硫酸化形式。
