Pahernik S A, Dellian M, Berr F, Tannapfel A, Wittekind C, Goetz A E
Institute for Surgical Research, University of Munich, Klinikum Grosshadern, Germany.
J Photochem Photobiol B. 1998 Nov;47(1):58-62. doi: 10.1016/s1011-1344(98)00203-6.
Prognosis of patients with bile duct tumors is mostly poor due to late diagnosis and a lack of adequate curative and palliative treatment modalities. To evaluate the potential of photodynamic therapy (PDT) as a novel and alternative treatment approach, we have investigated the uptake and tumor-specific localization of the photosensitizer Photofrin in human biliary tract neoplasms. We have quantified the distribution and the pharmacokinetics of Photofrin in normal and tumor tissue biopsies of the human bile duct by quantitative fluorescence microscopy and digital image analysis of cryosections. Fluorescence intensities (expressed as a percentage of a standard) are 19.0 +/- 11.4% and 25.2 +/- 12.7% for tumors and 10.9 +/- 2.9% and 13.2 +/- 9.1% (mean +/- SD) for normal bile duct tissue at 24 h (n = 5) and 48 h (n = 8) after Photofrin administration (2 mg kg-1 i.v.), respectively, and decrease afterwards in normal bile duct tissue over the period of investigation (4-35 days). The ratios of fluorescence in tumor versus normal tissue are found to be 1.7 +/- 0.7 and 2.3 +/- 1.2 (mean +/- SD) at days one and two after Photofrin administration, respectively. Thus, Photofrin preferentially accumulates in bile duct neoplasms, reaching peak values during the first two days. These data suggest that laser irradiation should be performed within this period after Photofrin injection to achieve tumor selectivity of PDT for effective treatment of bile duct carcinoma.
由于诊断较晚且缺乏足够的根治性和姑息性治疗方式，胆管肿瘤患者的预后大多较差。为了评估光动力疗法（PDT）作为一种新型替代治疗方法的潜力，我们研究了光敏剂卟吩姆钠在人胆道肿瘤中的摄取和肿瘤特异性定位。我们通过定量荧光显微镜和冰冻切片的数字图像分析，对人胆管正常组织活检和肿瘤组织活检中卟吩姆钠的分布及药代动力学进行了定量分析。在静脉注射卟吩姆钠（2 mg kg-1）后24小时（n = 5）和48小时（n = 8），肿瘤组织的荧光强度（以标准的百分比表示）分别为19.0 +/- 11.4%和25.2 +/- 12.7%，正常胆管组织的荧光强度分别为10.9 +/- 2.9%和13.2 +/- 9.1%（平均值 +/- 标准差），随后在研究期间（4 - 35天）正常胆管组织中的荧光强度下降。在注射卟吩姆钠后的第1天和第2天，肿瘤组织与正常组织的荧光强度比值分别为1.7 +/- 0.7和2.3 +/- 1.2（平均值 +/- 标准差）。因此，卟吩姆钠优先在胆管肿瘤中蓄积，在头两天达到峰值。这些数据表明，应在注射卟吩姆钠后的这段时间内进行激光照射，以实现光动力疗法对胆管癌的肿瘤选择性有效治疗。
