胸腺细胞的体外致敏。H-21 I区决定簇和无细胞混合白细胞培养上清液在细胞毒性反应产生中的作用。
In vitro sensitization of thymocytes. Role of H-21 I region determinants and cell-free mixed leukocyte culture supernates in generation of cytotoxic responses.
作者信息
Sopori M, Bernstein A, Bach F H
出版信息
J Exp Med. 1978 Oct 1;148(4):953-62. doi: 10.1084/jem.148.4.953.
Abstract
Stimulation of thymocytes in vitro by spleen cells differing for the entire H-2 complex leads to a significant proliferative response without a significant cell-mediated lympholysis (CML) response. Addition of soluble cell-free supernates (SF), (taken from a 7-day mixed leukocyte culture) enables these cultures to develop CML response. For optimal CML response, the SF has to be added within 48 h of onset of cultures. Although with spleen cells as responding cells, SF could quantitatively replace I-region different stimulating cells for generation of CML responses, with thymocytes as responding cells, stimulation with I-region cells appeared obligatory for the generation of CML responses. The implications of these findings are discussed.
摘要
用在整个H-2复合体上存在差异的脾细胞在体外刺激胸腺细胞，会引发显著的增殖反应，但不会产生显著的细胞介导的淋巴细胞溶解（CML）反应。添加可溶性无细胞上清液（SF，取自7天的混合白细胞培养物）可使这些培养物产生CML反应。为了获得最佳的CML反应，必须在培养开始后的48小时内添加SF。尽管以脾细胞作为反应细胞时，SF在数量上可以替代I区不同的刺激细胞来产生CML反应，但以胸腺细胞作为反应细胞时，I区细胞的刺激似乎是产生CML反应所必需的。本文讨论了这些发现的意义。
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