Lamsfus C, Jordana R, Ponz F
Rev Esp Fisiol. 1976 Dec;32(4):331-4.
Glucose intestine active transport in tortoise Testudo hermanni robertmertensi Wermuth has been studied in vitro in the presence of known sugar active transport inhibiting substances. The final Serosal/Mucosal gradient is practically the same in aerobic and anaerobic conditions. A 10(-4) M concentration of DNP inhibits active transport of glucose and increases O2 uptake; a 10(-3) M concentration reinforces transport inhibition and lowers O2 uptake to normal values. Nullification of glucose active transport was not achieved by any of the DNP essayed concentrations. NaF greatly inhibits both glucose active transport and O2 uptake, whereas phlorizin inhibits transport and does not affect respiration. Tortoise intestine is able to obtain the required energy for its glucose active transport through both aerobic and anaerobic metabolism. Besides its oxidative phosphorylation uncoupling action, DNP also appears to affect glycolysis. Glycolysis inhibition and intestinal epithelial alteration may be responsible for the strong inhibition caused by NaF. Phlorizin seems to inhibit sugar transport by competence on the sugar carrier, at membrane level, without disturbing cellular metabolism.
在已知的糖主动运输抑制物质存在的情况下，对赫曼陆龟（Testudo hermanni robertmertensi Wermuth）肠道中的葡萄糖主动运输进行了体外研究。在有氧和无氧条件下，最终的浆膜/黏膜梯度实际上是相同的。10⁻⁴M浓度的二硝基苯酚（DNP）抑制葡萄糖的主动运输并增加氧气摄取；10⁻³M浓度增强运输抑制并将氧气摄取降低到正常值。所试验的任何DNP浓度均未实现葡萄糖主动运输的无效化。氟化钠（NaF）极大地抑制葡萄糖主动运输和氧气摄取，而根皮苷抑制运输且不影响呼吸作用。陆龟肠道能够通过有氧和无氧代谢为其葡萄糖主动运输获得所需能量。除了其氧化磷酸化解偶联作用外，DNP似乎还影响糖酵解。糖酵解抑制和肠上皮改变可能是NaF引起强烈抑制的原因。根皮苷似乎通过在膜水平上作用于糖载体来抑制糖运输，而不会干扰细胞代谢。
