通过测量释放-摄取比率来测定存活人红细胞的钾通量活性。

Determination of potassium flux activity of viable human erythrocytes by measuring the release-influx ratio.

作者信息

Ozawa Y, Sato S, Matsumoto K, Nishi S, Shishido S, Imafuku Y, Miura Y, Yoshida H

机构信息

Department of Clinical Laboratory Medicine and Clinical Laboratories, Fukushima Medical University, Japan.

出版信息

Clin Chim Acta. 1999 Jan;279(1-2):125-32. doi: 10.1016/s0009-8981(98)00175-2.

DOI:10.1016/s0009-8981(98)00175-2

PMID:10064124

Abstract

A simple and convenient method to determine the K+-flux activity of viable human erythrocytes was developed. Erythrocyte suspensions were incubated at 4 degrees C for 24 h to induce K+-release (deltaKr) and then at 37 degrees C for 3 h to influx K+ into erythrocytes (deltaKi). A straight-line relationship between K+ release-influx ratio and ouabain-induced K+-efflux from erythrocytes indicated that deltaKi/deltaKr ratio or the K+-flux activity was reflected predominantly by Na+/K+-exchanging ATPase activity. Using this method, K+-flux activity of erythrocytes in the young and the aged subjects was measured. The mean deltaKi/deltaKr ratio of the aged subjects was decreased significantly. This method of measuring deltaKi/deltaKr ratio is useful for the evaluation of K+-flux activity of viable erythrocytes.

摘要

开发了一种简单便捷的方法来测定存活人红细胞的钾通量活性。将红细胞悬液在4℃下孵育24小时以诱导钾释放（δKr），然后在37℃下孵育3小时以使钾流入红细胞（δKi）。钾释放-流入比与哇巴因诱导的红细胞钾外流之间的直线关系表明，δKi/δKr比或钾通量活性主要由钠/钾交换ATP酶活性反映。使用该方法，测量了年轻和老年受试者红细胞的钾通量活性。老年受试者的平均δKi/δKr比显著降低。这种测量δKi/δKr比的方法对于评估存活红细胞的钾通量活性很有用。