Bradlow B A, Gomperts E D, Hockley J
S Afr Med J. 1976 Nov 20;50(50):1989-92.
As part of an international co-operative study of the International Committee for Standardisation in Haematology, human brain thromboplastins prepared by the acetone-extracted and phenol-saline extraction techniques have been compared as to the yield, the proportion of reagent discarded and wasted, and the sensitivity related to an international standard, the British Comparative Thromboplastin standard (BCT). The phenol-saline extract showed greatly improved sensitivity with greatly reduced batch-to-batch variation as compared with the acetone-dried preparation. In addition, the sensitivity of this reagent conformed to that of the BCT. The implications of the use of such a standardised reagent to the therapeutic management of oral anticoagulant therapy are: (i) standardised, reproducible results; (ii) results comparable with those carried out by other laboratories using a similarly standardised reagent; and (iii) stable therapeutic range.
作为国际血液学标准化委员会国际合作研究的一部分，对通过丙酮萃取和酚盐萃取技术制备的人脑凝血活酶在产量、试剂废弃和浪费比例以及与国际标准（英国比较凝血活酶标准，BCT）相关的灵敏度方面进行了比较。与丙酮干燥制剂相比，酚盐提取物显示出灵敏度大大提高，批次间差异大大降低。此外，该试剂的灵敏度符合BCT的灵敏度。使用这种标准化试剂对口服抗凝治疗的治疗管理的意义在于：（i）标准化、可重复的结果；（ii）结果可与其他使用类似标准化试剂的实验室进行的结果相比较；（iii）稳定的治疗范围。
