Morgan L, Kumaresan S, Thomas C, MacWilliams P
School of Veterinary Medicine, University of Wisconsin-Madison, 53706, USA.
J Zoo Wildl Med. 1998 Dec;29(4):394-400.
Most reported laboratory reference values for harbor seals (Phoca vitulina) are derived from captive seals, or stranded seals that have recovered from disease in marine mammal centers. This study established hematology and serum chemistry reference values for free-ranging harbor seals, using methods and that are current and readily available, and determined the effects of hemolysis on serum chemistry values of captive harbor seals. Blood samples were collected for hematologic and serum chemistry measurements from 14 clinically normal, adult male and female harbor seals and two juvenile harbor seals (approximate age 6 mo) captured in saltwater sloughs and estuaries near Moss Landing, California, USA. Values for amylase, globulin, and differential leukocyte count, not previously reported, were determined. In general, hematology and chemistry values in adults were similar to those reported for free-ranging and captive harbor seals, except for glucose, urea nitrogen, and lactate dehydrogenase (LDH) values, which were higher than those reported previously. Red blood cell counts in the two juveniles were higher than in adults and in young harbor seals studied previously. To determine the effects of hemolysis on serum chemistry values, two intensities of hemolysis were generated experimentally in blood collected from 11 harbor seals recovering from injuries or stranding at the Marine Mammal Center (Sausalito, California 94965, USA). Moderate hemolysis (++, 1 g/L hemoglobin, red-tinged) significantly increased LDH activity, whereas severe hemolysis ( , 2 g/L hemoglobin, cherry red) significantly increased total protein, albumin, calculated globulin, LDH, and total bilirubin and significantly decreased creatinine. The effects of hemolysis must be considered when chemistry results of harbor seals are interpreted.
大多数报道的斑海豹（Phoca vitulina）实验室参考值来自圈养海豹，或在海洋哺乳动物中心从疾病中康复的搁浅海豹。本研究采用当前可用的方法，为自由放养的斑海豹建立了血液学和血清化学参考值，并确定了溶血对圈养斑海豹血清化学值的影响。从14只临床正常的成年雄性和雌性斑海豹以及2只幼年斑海豹（约6个月大）采集血液样本进行血液学和血清化学测量，这些海豹是在美国加利福尼亚州莫斯兰丁附近的盐水泻湖和河口捕获的。测定了淀粉酶、球蛋白和白细胞分类计数的值，这些值以前未曾报道过。一般来说，成年海豹的血液学和化学值与自由放养和圈养斑海豹报道的值相似，但葡萄糖、尿素氮和乳酸脱氢酶（LDH）值高于以前报道的值。两只幼年海豹的红细胞计数高于成年海豹以及先前研究的幼年斑海豹。为了确定溶血对血清化学值的影响，在从美国加利福尼亚州索萨利托海洋哺乳动物中心（邮编94965）11只从受伤或搁浅中恢复的斑海豹采集的血液中，通过实验产生了两种溶血强度。中度溶血（++，血红蛋白1 g/L，略带红色）显著增加LDH活性，而重度溶血（ ，血红蛋白2 g/L，樱桃红色）显著增加总蛋白、白蛋白、计算得出的球蛋白、LDH和总胆红素，并显著降低肌酐。在解释斑海豹的化学检测结果时，必须考虑溶血的影响。
