Lander Michelle E, Harvey James T, Gulland Frances M
Moss Landing Marine Laboratories, 8272 Moss Landing Road, Moss Landing, California 95039, USA.
J Wildl Dis. 2003 Jul;39(3):600-9. doi: 10.7589/0090-3558-39.3.600.
The objectives of this study were to compare the hematology and serum chemistry values between free-ranging and stranded harbor seal (Phoca vitulina richardsi) pups and to ascertain how blood values of stranded pups changed during the rehabilitation process. Coincident with these comparisons, reference values were obtained for free-ranging pups. Stranded harbor seal pups (n = 28) recovered from areas between Pebble Beach and Moss Landing, California (USA) were admitted to The Marine Mammal Center, Sausalito, from March to May 1995, 1996, and 1998. Blood samples were collected from harbor seal pups before and after rehabilitation. As a control group, wild harbor seal pups were captured at Pebble Beach and Elkhorn Slough (n = 42) during the 1995, 1996, and 1998 pupping seasons. Mean eosinophil and calcium values of wild pups were significantly greater than those of newly admitted pups, whereas mean bands, aspartate aminotransferase, alanine aminotransferase, total bilirubin, and chloride values were significantly lower (P < or = 0.05). Mean neutrophil, band, lymphocyte, eosinophil, basophil, calcium, phosphorus, blood urea nitrogen, potassium, total protein, and globulin values of rehabilitated pups increased significantly after 2-3 mo in captivity, whereas, mean red blood cell, hemoglobin, hematocrit, cholesterol, and total bilirubin values decreased significantly (P < or = 0.05).
本研究的目的是比较自由放养的和搁浅的港海豹（理查兹港海豹，Phoca vitulina richardsi）幼崽的血液学和血清化学值，并确定搁浅幼崽在康复过程中血液值是如何变化的。在进行这些比较的同时，还获得了自由放养幼崽的参考值。1995年、1996年和1998年3月至5月期间，从美国加利福尼亚州卵石滩和莫斯兰丁之间区域获救的搁浅港海豹幼崽（n = 28）被送往索萨利托的海洋哺乳动物中心。在康复前后从港海豹幼崽身上采集血样。作为对照组，在1995年、1996年和1998年的产仔季节，在卵石滩和埃尔克霍恩湿地捕获野生港海豹幼崽（n = 42）。野生幼崽的平均嗜酸性粒细胞和钙值显著高于新入院的幼崽，而平均杆状核细胞、天冬氨酸转氨酶、丙氨酸转氨酶、总胆红素和氯值则显著较低（P≤0.05）。被圈养2 - 3个月后，康复幼崽的平均中性粒细胞、杆状核细胞、淋巴细胞、嗜酸性粒细胞、嗜碱性粒细胞、钙、磷、血尿素氮、钾、总蛋白和球蛋白值显著升高，而平均红细胞、血红蛋白、血细胞比容、胆固醇和总胆红素值则显著降低（P≤0.05）。
