Jang I, Jung K, Cho J
Laboratory Animal Resources, Korea FDA, Seoul, Korea.
Exp Anim. 1998 Oct;47(4):247-52. doi: 10.1538/expanim.47.247.
The present study was designed to determine age-related changes in intestinal and hepatic antioxidant enzymes including superoxide dismutase (SOD), glutathione peroxidase (GSH-PX), and glutathione-S-transferase (GST), and lipid peroxidation in male Wistar rats (n = 8) aged 2 wk, 2.5 mon, 5 mon, 10 mon, and 23 mon. In the small intestine, cytosolic SOD, GSH-PX activities and lipid peroxidation were not affected by age, but intestinal GST activity was noticeably enhanced as age increased. In particular, intestinal GST activity in 23 mon old rats was 3 times as strong as that in 2 wk old rats. In the liver, the activity of hepatic cytosolic SOD was not affected by age, whereas GSH-PX and GST activities in rats aged 10 mon and 23 mon were much stronger than those in rats aged 2 wk, 2.5 mon, and 5 mon. The increased lipid peroxidation in 2.5 mon and 5 mon old rats was observed when compared with that of other groups. It is therefore concluded from the results presented here that age greatly increases GST activity in the small intestinal mucosae and increasing GSH-PX, GST activities and lipid peroxidation in the liver from male Wistar rats.
本研究旨在确定雄性Wistar大鼠（n = 8）在2周龄、2.5月龄、5月龄、10月龄和23月龄时，肠道和肝脏中抗氧化酶（包括超氧化物歧化酶（SOD）、谷胱甘肽过氧化物酶（GSH-PX）和谷胱甘肽-S-转移酶（GST））以及脂质过氧化的年龄相关变化。在小肠中，胞质SOD、GSH-PX活性和脂质过氧化不受年龄影响，但肠道GST活性随年龄增加而显著增强。特别是，23月龄大鼠的肠道GST活性是2周龄大鼠的3倍。在肝脏中，肝脏胞质SOD活性不受年龄影响，而10月龄和23月龄大鼠的GSH-PX和GST活性比2周龄、2.5月龄和5月龄大鼠强得多。与其他组相比，2.5月龄和5月龄大鼠的脂质过氧化增加。因此，根据此处给出的结果得出结论，年龄会极大地增加雄性Wistar大鼠小肠黏膜中的GST活性，并增加其肝脏中的GSH-PX、GST活性和脂质过氧化。
