Jang I, Chae K, Cho J
Department of Animal Science and Biotechnology, Chinju National University 150, Chilam-Dong, Chinju, 660-758, Kyung-Nam, South Korea.
Mech Ageing Dev. 2001 May 15;122(6):561-70. doi: 10.1016/s0047-6374(01)00236-6.
Age- and strain-associated alterations in intestinal and hepatic antioxidant defense enzymes including superoxide dismutase (SOD), glutathione peroxidase (GSH-PX), and glutathione-S-transferase (GST), and lipid peroxidation were examined in Wistar and F344 rats of both strains aged 2 weeks, 2.5, 10 and 23 months. In the small intestine, activities of SOD and GSH-PX and lipid peroxidation were not affected by age or strain difference. Intestinal GST activity was noticeably increased with age in both strains, but somewhat different pattern of age-related changes occurred between two strains. Wistar rats aged 23 months had a significantly higher intestinal GST activity than corresponding age of F344 rats. In the liver, cytosolic SOD activity was not affected by age and strain, whereas GSH-PX and GST activities and lipid peroxidation were markedly influenced by age or strain difference. In particular, hepatic GSH-PX in Wistar rats resulted in a significant increase after 10 months of age and stayed at this level till 23 months of age we examined. Also, Wistar rats showed a higher lipid peroxidation in the liver of 2.5 months old when compared with corresponding age of F344 rats. However, F344 rats did not show any significant age-dependent changes in GSH-PX and lipid peroxidation. In contrast, the GST activity did show much of an age-associated alteration in both strains. Age-associated change in GST activity of Wistar rats was much greater than that observed in F344 rats, especially late in the lifetime (23 months old). It is concluded from our results that age has profound impact on development of some antioxidant enzymes in the small intestine and liver and also strain-related difference in development of antioxidant defense system was observed at least some time of rat life.
在2周龄、2.5月龄、10月龄和23月龄的Wistar大鼠和F344大鼠中，研究了年龄和品系相关的肠道和肝脏抗氧化防御酶（包括超氧化物歧化酶（SOD）、谷胱甘肽过氧化物酶（GSH-PX）和谷胱甘肽-S-转移酶（GST））的变化以及脂质过氧化情况。在小肠中，SOD和GSH-PX的活性以及脂质过氧化不受年龄或品系差异的影响。两个品系的肠道GST活性均随年龄显著增加，但两个品系之间年龄相关变化的模式略有不同。23月龄的Wistar大鼠肠道GST活性显著高于相应年龄的F344大鼠。在肝脏中，胞质SOD活性不受年龄和品系的影响，而GSH-PX和GST活性以及脂质过氧化则受到年龄或品系差异的显著影响。特别是，Wistar大鼠肝脏中的GSH-PX在10月龄后显著增加，并在我们检测的23月龄时一直保持在该水平。此外，与相应年龄的F344大鼠相比，2.5月龄的Wistar大鼠肝脏中的脂质过氧化更高。然而，F344大鼠在GSH-PX和脂质过氧化方面未显示出任何显著的年龄依赖性变化。相比之下，两个品系的GST活性均显示出与年龄相关的变化。Wistar大鼠GST活性的年龄相关变化远大于F344大鼠，尤其是在生命后期（23月龄）。我们的研究结果表明，年龄对小肠和肝脏中某些抗氧化酶的发育有深远影响，并且在大鼠生命的至少某些阶段观察到了抗氧化防御系统发育中的品系相关差异。
