Meng Z Q, Zhang L Z
Department of Life Sciences, Shanxi University, Taiyuan.
Yi Chuan Xue Bao. 1998;25(4):294-300.
In this paper, the single cell microgel electrophoresis (SCG) technique was described in detail, and effects of DNA damage of human blood lymphocytes induced by gamma-rays radiation, H2O2 and CdCl2 were studied using this technique. It was shown that gamma-rays, H2O2 and CdCl2 all caused the increase of DNA migration length in the lymphocytes in the dose-dependent manner. The principle of the SCG technique, cause of DNA migration in the untreated control cells, and noticeable details in the SCG technique were also discussed.
本文详细描述了单细胞微凝胶电泳（SCG）技术，并使用该技术研究了γ射线辐射、H2O2和CdCl2诱导的人血淋巴细胞DNA损伤效应。结果表明，γ射线、H2O2和CdCl2均以剂量依赖方式导致淋巴细胞中DNA迁移长度增加。还讨论了SCG技术的原理、未处理对照细胞中DNA迁移的原因以及SCG技术中的显著细节。
