Abaru D E, Denham D A
Trans R Soc Trop Med Hyg. 1976;70(4):333-4. doi: 10.1016/0035-9203(76)90091-2.
The technique involves taking a measured volume of blood (20-40 mm3) from a finger or ear prick on to a conventional 3 X 1 in microscope slide and lysing it with water. Microfilariae could either be counted immediately, whilst still alive, or the smear dried, fixed and stained. A comparison between counts of microfilariae in freshly prepared slides and stained slides showed that few microfilariae were lost in the staining process.
该技术包括从手指或耳垂穿刺采集一定体积（20 - 40立方毫米）的血液，滴在常规的3×1英寸显微镜载玻片上，然后用水使其裂解。微丝蚴可以在仍存活时立即计数，或者将涂片干燥、固定并染色。新鲜制备的载玻片和染色载玻片上微丝蚴计数的比较表明，在染色过程中很少有微丝蚴丢失。
