Grove D I, Davis R S, Warren K S
Parasitology. 1979 Dec;79(3):303-16. doi: 10.1017/s0031182000053713.
Microfilariae of Brugia malayi were obtained from the peritoneal cavities of infected gerbils and were then injected intravenously into mice. A sub-periodic, nocturnal microfilaraemia was produced. The level of microfilaraemia was proportional to the number of parasites injected, with approximately 1-3% of microfilariae being found in the peripheral circulation. The duration of microfilaraemia was proportional to the number of parasites injected; it subsided by 30 days after injection of 104 microfilariae but was still present at a low level 120 days after injection of 2 x 105 microfilariae. A transient splenomegaly developed after injection of microfilariae. Histopathological examination revealed large numbers of microfilariae free in the lumens of pulmonary small blood vessels and without any accompanying inflammatory reaction. Lesser numbers of microfilariae were seen in the cardiac blood and hepatic and renal blood vessels for the first few days after injection. There was cellular proliferation in the splenic white pulp and vascular congestion of the red pulp. Microfilariae labelled with 51Cr were injected intravenously; 57% of radioactivity was found in the lungs, 8.5% in the liver and 2.9% in the spleen. Mice developed immediate hypersensitivity reactions to B. malayi antigen by 4 weeks after injection, but Arthus and delayed hypersensitivity reactions were not seen at any time. when mice which had been injected 5 months previously were challenged with a 2nd injection of microfilariae, there was an accelerated clearance of parasites over 2 weeks and a marked peripheral blood eosinophilia developed. In contrast with natural infections, in which the continuous production of microfilariae complicates assessment, this model provides a system in which factors controlling the circulation of microfilariae in the bloodstream can be studied independently.
马来布鲁线虫微丝蚴取自感染沙鼠的腹腔，然后静脉注射到小鼠体内。产生了亚周期型夜间微丝蚴血症。微丝蚴血症的水平与注射的寄生虫数量成正比，在外周循环中约可发现1% - 3%的微丝蚴。微丝蚴血症的持续时间与注射的寄生虫数量成正比；注射104条微丝蚴后30天微丝蚴血症消退，但注射2×105条微丝蚴后120天仍有低水平存在。注射微丝蚴后出现短暂性脾肿大。组织病理学检查显示，肺小血管腔内有大量游离的微丝蚴，且无任何伴随的炎症反应。注射后的头几天，在心脏血液以及肝和肾血管中可见较少数量的微丝蚴。脾白髓有细胞增殖，红髓有血管充血。静脉注射用51Cr标记的微丝蚴；57%的放射性出现在肺部，8.5%在肝脏，2.9%在脾脏。注射后4周小鼠对马来布鲁线虫抗原产生即刻超敏反应，但在任何时候均未见到阿瑟斯反应和迟发型超敏反应。当5个月前注射过的小鼠再次注射微丝蚴时，2周内寄生虫清除加速，并出现明显的外周血嗜酸性粒细胞增多。与自然感染不同，自然感染中微丝蚴的持续产生使评估复杂化，而该模型提供了一个系统，在其中可以独立研究控制微丝蚴在血流中循环的因素。
