Nakashima Y, Sun D H, Trindade M C, Chun L E, Song Y, Goodman S B, Schurman D J, Maloney W J, Smith R L
Stanford University School of Medicine, California, USA.
J Bone Joint Surg Br. 1999 Jan;81(1):155-62. doi: 10.1302/0301-620x.81b1.8884.
Particulate wear debris is associated with periprosthetic inflammation and loosening in total joint arthroplasty. We tested the effects of titanium alloy (Ti-alloy) and PMMA particles on monocyte/macrophage expression of the C-C chemokines, monocyte chemoattractant protein-1 (MCP-1), monocyte inflammatory protein-1 alpha (MIP-1alpha), and regulated upon activation normal T expressed and secreted protein (RANTES). Periprosthetic granulomatous tissue was analysed for expression of macrophage chemokines by immunohistochemistry. Chemokine expression in human monocytes/macrophages exposed to Ti-alloy and PMMA particles in vitro was determined by RT-PCR, ELISA and monocyte migration. We observed MCP-1 and MIP-1alpha expression in all tissue samples from failed arthroplasties. Ti-alloy and PMMA particles increased expression of MCP-1 and MIP-1alpha in macrophages in vitro in a dose- and time-dependent manner whereas RANTES was not detected. mRNA signal levels for MCP-1 and MIP-1alpha were also observed in cells after exposure to particles. Monocyte migration was stimulated by culture medium collected from macrophages exposed to Ti-alloy and PMMA particles. Antibodies to MCP-1 and MIP-1alpha inhibited chemotactic activity of the culture medium samples. Release of C-C chemokines by macrophages in response to wear particles may contribute to chronic inflammation at the bone-implant interface in total joint arthroplasty.
颗粒状磨损碎屑与全关节置换术中假体周围炎症和松动有关。我们测试了钛合金（Ti合金）和聚甲基丙烯酸甲酯（PMMA）颗粒对单核细胞/巨噬细胞中C-C趋化因子、单核细胞趋化蛋白-1（MCP-1）、单核细胞炎性蛋白-1α（MIP-1α）以及活化后正常T细胞表达和分泌蛋白（RANTES）表达的影响。通过免疫组织化学分析假体周围肉芽肿组织中巨噬细胞趋化因子的表达。通过逆转录聚合酶链反应（RT-PCR）、酶联免疫吸附测定（ELISA）和单核细胞迁移来确定体外暴露于Ti合金和PMMA颗粒的人单核细胞/巨噬细胞中的趋化因子表达。我们在失败的关节置换术的所有组织样本中观察到了MCP-1和MIP-1α的表达。Ti合金和PMMA颗粒在体外以剂量和时间依赖性方式增加了巨噬细胞中MCP-1和MIP-1α的表达，而未检测到RANTES。在暴露于颗粒后的细胞中也观察到了MCP-1和MIP-1α的mRNA信号水平。暴露于Ti合金和PMMA颗粒的巨噬细胞收集的培养基刺激了单核细胞迁移。针对MCP-1和MIP-1α的抗体抑制了培养基样本的趋化活性。巨噬细胞对磨损颗粒作出反应而释放C-C趋化因子可能导致全关节置换术中骨-植入物界面的慢性炎症。
