Serwer P, Griess G A
Department of Biochemistry, The University of Texas Health Science Center, San Antonio 78284-7760, USA.
J Chromatogr B Biomed Sci Appl. 1999 Feb 5;722(1-2):179-90. doi: 10.1016/s0378-4347(98)00404-6.
Nondenaturing gel electrophoresis is used to both characterize multimolecular particles and determine the assembly pathways of these particles. Characterization of bacteriophage-related particles has yielded strategies for characterizing multimolecular particles in general. Previous studies have revealed means for using nondenaturing gel electrophoresis to determine both the effective radius and the average electrical surface charge density of any particle. The response of electrophoretic mobility to increasing the magnitude of the electrical field is used to detect rod-shaped particles. To increase the capacity of nondenaturing gel electrophoresis to characterize comparatively large particles, some current research is directed towards either determining the structure of gels used for electrophoresis or inducing steric trapping of particles in dead-end regions within the fibrous network that forms a gel. A trapping-dependent technique of pulsed-field gel electrophoresis is presented with which a DNA-protein complex can be made to electrophoretically migrate in a direction opposite to the direction of migration of protein-free DNA.
非变性凝胶电泳用于表征多分子颗粒并确定这些颗粒的组装途径。对噬菌体相关颗粒的表征已产生了一般表征多分子颗粒的策略。先前的研究揭示了使用非变性凝胶电泳来确定任何颗粒的有效半径和平均电表面电荷密度的方法。电泳迁移率对电场强度增加的响应被用于检测棒状颗粒。为了提高非变性凝胶电泳表征相对较大颗粒的能力，目前一些研究致力于确定用于电泳的凝胶结构，或诱导颗粒在形成凝胶的纤维网络内的死端区域中发生空间捕获。本文介绍了一种依赖捕获的脉冲场凝胶电泳技术，利用该技术可使DNA-蛋白质复合物在电泳中朝着与无蛋白质DNA迁移方向相反的方向移动。
