Santini G, De Pirro M, Chelazzi G
Consorzio Nazionale Interuniversitario per le Scienze del Mare, c/o Dipartimento di Biologia Animale e Genetica, Università degli Studi di Firenze, Via Romana 17, I-50125 Firenze, Italy. gsantini@dbag. unifi.it
Physiol Biochem Zool. 1999 Mar-Apr;72(2):198-204. doi: 10.1086/316656.
Heart rate of the Mediterranean limpet Patella caerulea L. was investigated on the natural shore and in the laboratory by using a technique based on infrared phototransducers. Field recording occurred in the Gulf of Trieste (northern Adriatic) during March and June 1997. A consistent dependence of heart rate on temperature was observed in limpets both when submerged and when exposed to air in the two periods, but thermal acclimation was evident. During spontaneous activity at high tide, heart rate increased 1.5-1.7 times the values observed during resting in water at corresponding temperatures. The dependence of heart rate on temperature (10 degrees, 16 degrees, and 22 degrees C) and size (wet weight <1.25 and >1.30 g) in submerged limpets from different populations (northern Adriatic and Tyrrhenian) was tested in the laboratory by adopting a factorial design. The results showed a marked effect of temperature, body weight, and their interaction, independent from the site of origin. Smaller limpets showed a linear increase of heart rate in the whole range of temperature tests, while in the larger ones the increase between 10 degrees and 16 degrees C was greater than between 16 degrees and 22 degrees C. Heart rate decreased with increasing body size at control (16 degrees C) and high (22 degrees C) temperature, while at lower temperature (10 degrees C) no effect of body size was evident. When removed from their home scar, limpets increased heart rate to about 1.5 times the reference value. Finally, correlation of oxygen consumption with heart rate of submerged limpets maintained at a different temperature (10 degrees -22 degrees C) was statistically significant.
采用基于红外光电传感器的技术，在地中海滨螺（Patella caerulea L.）的自然栖息地和实验室中对其心率进行了研究。实地记录于1997年3月和6月在的里雅斯特湾（亚得里亚海北部）进行。在这两个时期，无论是在水中还是暴露于空气中，都观察到滨螺的心率与温度之间存在一致的相关性，但热适应现象明显。在涨潮时的自发活动期间，心率增加到相应温度下在水中静息时观察值的1.5 - 1.7倍。通过采用析因设计，在实验室中测试了来自不同种群（亚得里亚海北部和第勒尼安海）的水下滨螺的心率对温度（10℃、16℃和22℃）和大小（湿重<1.25克和>1.30克）的依赖性。结果表明，温度、体重及其相互作用具有显著影响，与来源地无关。较小的滨螺在整个温度测试范围内心率呈线性增加，而较大的滨螺在10℃至16℃之间的增加幅度大于在16℃至22℃之间的增加幅度。在对照温度（16℃）和高温（22℃）下，心率随体重增加而降低，而在低温（10℃）下，体重对心率没有明显影响。当从其栖息地移除时，滨螺的心率增加到参考值的约1.5倍。最后，在不同温度（10℃ - 22℃）下维持的水下滨螺的耗氧量与心率之间的相关性具有统计学意义。
