Sun Yong-Xu, Hu Li-Sha, Dong Yun-Wei
State Key Laboratory of Marine Environmental Science, College of Ocean and Earth Sciences, Xiamen University, Xiamen 361102, China.
Key Laboratory of Mariculture, Ministry of Education, Fisheries College, Ocean University of China, Qingdao 266003, China.
iScience. 2023 Feb 3;26(3):106128. doi: 10.1016/j.isci.2023.106128. eCollection 2023 Mar 17.
High-throughput determination of circadian rhythms in metabolic response and their divergent patterns in various microhabitats are crucial for understanding how organisms respond to environmental stresses. A mid-intertidal limpet was collected at various time points across both daytime and nighttime in winter during low tide for investigating the diurnal metabolomic responses to cold stress and elucidating the divergent metabolic responses to temperature variations across microhabitats. Temperatures of emergent rock microhabitats were lower than the tidal pool and even aggravated at night. A series of metabolomic responses exhibited coordinated diurnal changes in winter. Metabolic responses which were associated with cellular stress responses and energy metabolism of emergent rock microhabitat individuals were highly induced compared to the tidal pool ones. This study shed light on the diurnal patterns of metabolomic responses of intertidal molluscs in the field and emphasized the variations in metabolic responses between microhabitats.
高通量测定代谢反应中的昼夜节律及其在各种微生境中的不同模式，对于理解生物体如何应对环境压力至关重要。在冬季低潮期的白天和夜间的不同时间点采集潮间带帽贝，以研究其对冷应激的昼夜代谢组学反应，并阐明跨微生境对温度变化的不同代谢反应。暴露岩石微生境的温度低于潮汐池，且在夜间甚至加剧。一系列代谢组学反应在冬季呈现出协调的昼夜变化。与潮汐池个体相比，与暴露岩石微生境个体的细胞应激反应和能量代谢相关的代谢反应被高度诱导。这项研究揭示了潮间带软体动物在野外的代谢组学反应的昼夜模式，并强调了微生境之间代谢反应的差异。
