Aboul-Enein H Y, Stefan R I, Radu G L
Bioanalytical and Drug Development Laboratory, Biological and Medical Research Department, King Faisal Specialist Hospital and Research Centre, Riyadh, Saudi Arabia.
Prep Biochem Biotechnol. 1999 Feb;29(1):55-61. doi: 10.1080/10826069908544693.
Because S-perindopril enantiomer is the eutomer which is responsible for the angiotensin-converting enzyme inhibition activity, it is necessary to develop a reliable method for its assay from its distomer, the R-enantiomer. For this purpose, an amperometric biosensor was developed based on L-amino acid oxidase. The working range of the described biosensor was 20pmol/L-10 micromol/L on the 7.0-7.4 pH range, with a detection limit of 2pmol/L. The low enantioselectivity for R-perindopril, as compared with S-enantiomer, was demonstrated by both mixed solutions and separate solutions methods (amperometric selectivity coefficient is 1.0 x 10(-4)). The biosensor was also selective towards D-proline and polyvinylpyrrolidone. The amperometric biosensor can be used for enantioselective analysis of S-perindopril in raw material, with an RSD < 1%. The life time (t95%) of the biosensor is three weeks.
由于S-培哚普利对映体是负责血管紧张素转换酶抑制活性的优映体，因此有必要开发一种可靠的方法，从其差向异构体R-对映体中测定它。为此，基于L-氨基酸氧化酶开发了一种安培生物传感器。所述生物传感器在pH值7.0 - 7.4范围内的工作范围为20pmol/L - 10μmol/L，检测限为2pmol/L。通过混合溶液和单独溶液方法均证明了该生物传感器对R-培哚普利的对映选择性低于S-对映体（安培选择性系数为1.0×10(-4)）。该生物传感器对D-脯氨酸和聚乙烯吡咯烷酮也具有选择性。该安培生物传感器可用于原料药中S-培哚普利的对映选择性分析，相对标准偏差<1%。该生物传感器的寿命（t95%）为三周。
