van Staden J F, Stefan R I, Aboul-Enein H Y
Department of Chemistry, University of Pretoria, South Africa.
Fresenius J Anal Chem. 2000 May;367(2):178-80. doi: 10.1007/s002160051620.
A new amperometric biosensor based on D-aminoacid oxidase is described for the assay of R-perindopril. R-perindopril can be determined in the 400-20 nmol/L concentration range; the detection limit is 10 nmol/L. The selectivity was checked with S-perindopril, D- and L-proline, and polyvinylpyrrolidone. The main interfering species was D-proline. An automated system for the assay of R-perindopril based on the concept of flow injection with an amperometric biosensor (based on D-aminoacid oxidase) as detector is also described. The system is suitable for the on-line monitoring of R-perindopril at a sampling rate of 72 samples/h, in the linear range: 100 nmol/L -20 nmol/L with an RSD better than 0.09% (n = 10).
描述了一种基于D-氨基酸氧化酶的新型安培生物传感器，用于测定R-培哚普利。R-培哚普利的测定浓度范围为400 - 20 nmol/L；检测限为10 nmol/L。用S-培哚普利、D-和L-脯氨酸以及聚乙烯吡咯烷酮检查了选择性。主要干扰物质是D-脯氨酸。还描述了一种基于流动注射概念的自动化系统，该系统以安培生物传感器（基于D-氨基酸氧化酶）作为检测器来测定R-培哚普利。该系统适用于以72个样品/小时的采样速率在线监测R-培哚普利，线性范围为：100 nmol/L - 20 nmol/L，相对标准偏差优于0.09%（n = 10）。
