Stiebel-Kalish H, Gaton D D, Weinberger D, Loya N, Schwartz-Ventik M, Solomon A
Department of Ophthalmology, Rabin Medical Center, Petah Tiqva, Israel.
Eye (Lond). 1998;12 ( Pt 5):829-33. doi: 10.1038/eye.1998.213.
An animal model study was conducted to compare the efficacy of recurrent topical applications of hyaluronic acid and gentamicin ointment for the treatment of noninfected, mechanical corneal erosions.
An artificial, controlled wound of identical size and depth was inflicted to the corneas of three groups of rabbit eyes in order to measure their healing rates. One group was treated with hyaluronic acid (10 eyes) while the second group received gentamicin ointment (10 eyes). The third group remained untreated and served as the control (10 eyes). The rate of re-epithelisation was measured at 8-h intervals until complete re-epithelisation was observed. After complete wound closure, the rabbits were killed, and comparative histological examinations were performed.
Rabbit eyes treated with hyaluronic acid showed a significantly enhanced rate of epithelial defect closure compared with untreated eyes and a similar rate to that achieved with gentamicin ointment. In the eyes treated with hyaluronic acid a normal, multilayered epithelium was observed 48 h after complete healing, whereas the gentamicin-treated eyes showed an imperfectly layered epithelium, with irregularity of the cuboidal cells.
While both hyaluronic acid and gentamicin enhance corneal epithelial healing at comparable rates, our study suggests that hyaluronic acid may have a more favourable effect on the structure of the healing epithelium, and can offer an alternative mode of therapy for non-infectious corneal erosions.
进行一项动物模型研究，以比较透明质酸和庆大霉素眼膏反复局部应用治疗非感染性机械性角膜上皮糜烂的疗效。
对三组兔眼的角膜造成大小和深度相同的人工可控伤口，以测量其愈合速度。一组用透明质酸治疗（10只眼），第二组用庆大霉素眼膏治疗（10只眼）。第三组不治疗作为对照（10只眼）。每隔8小时测量一次上皮再形成率，直至观察到完全上皮再形成。伤口完全闭合后，处死兔子并进行组织学对比检查。
与未治疗的眼睛相比，用透明质酸治疗的兔眼上皮缺损闭合速度明显加快，且与用庆大霉素眼膏治疗的速度相似。在完全愈合48小时后，用透明质酸治疗的眼睛观察到正常的多层上皮，而用庆大霉素治疗的眼睛上皮分层不完善，立方体细胞不规则。
虽然透明质酸和庆大霉素均能以相似的速度促进角膜上皮愈合，但我们的研究表明，透明质酸可能对愈合上皮的结构有更有利的影响，可为非感染性角膜糜烂提供一种替代治疗方式。
