Dillon R J, Lane R P
Department of Biology and Biochemistry, University of Bath.
Parasitology. 1999 Jan;118 ( Pt 1):27-32. doi: 10.1017/s0031182098003588.
Binding to the midgut microvillar surface in the sandfly Phlebotomus papatasi is a prerequisite for successful development of Leishmania major within the gut of the vector. This paper describes a method for detecting microvillar-associated proteins which act as ligands for the parasite surface glycoconjugate lipophosphoglycan (LPG). Adhesion of LPG to midgut proteins was visualized by probing midgut extracts with LPG using a Western ligand blotting technique. Procyclic L. major LPG bound to a microvillar polypeptide band of 65 kDa (estimated in the non-reduced state) and bound variably to several lower molecular weight bands, probably degradation products or subunits of the primary binding polypeptides. Specificity of binding was confirmed by co-incubating biotinylated LPG with an LPG-specific mAb which resulted in a great reduction in binding.
在白蛉（巴氏白蛉）中与中肠微绒毛表面结合是硕大利什曼原虫在载体肠道内成功发育的先决条件。本文描述了一种检测作为寄生虫表面糖缀合物脂磷壁酸（LPG）配体的微绒毛相关蛋白的方法。使用蛋白质免疫印迹技术，通过用LPG探测中肠提取物来观察LPG与中肠蛋白的粘附情况。前鞭毛体硕大利什曼原虫LPG与一条65 kDa的微绒毛多肽带结合（在非还原状态下估计），并与几条较低分子量的带可变结合，可能是主要结合多肽的降解产物或亚基。通过将生物素化的LPG与LPG特异性单克隆抗体共同孵育，导致结合显著减少，从而证实了结合的特异性。
