Apffel A, Yin H, Hancock W S, McManigill D, Frenz J, Wu S L
Hewlett-Packard Laboratories, Palo Alto, CA 94304, USA.
J Chromatogr A. 1999 Feb 5;832(1-2):149-63. doi: 10.1016/s0021-9673(98)00957-1.
A system is described which allows operation of a range of capillary based liquid phase separations including capillary electrophoresis, isocratic and gradient capillary electrochromatography, isocratic and gradient capillary liquid chromatography and electrically assisted gradient capillary liquid chromatography. The system was coupled to electrospray ionization mass spectrometry in the electrically assisted capillary liquid chromatography mode to investigate the effect of applied voltage on the selectivity in peptide mapping separations. Analyses were performed on tryptic digests of recombinant human growth hormone and tissue plasminogen activator. The results show a small but useful effect on selectivity that can be used to fine tune specific separations.
本文描述了一种系统，该系统可实现一系列基于毛细管的液相分离操作，包括毛细管电泳、等度和梯度毛细管电色谱、等度和梯度毛细管液相色谱以及电辅助梯度毛细管液相色谱。该系统在电辅助毛细管液相色谱模式下与电喷雾电离质谱联用，以研究施加电压对肽图谱分离中选择性的影响。对重组人生长激素和组织纤溶酶原激活剂的胰蛋白酶消化产物进行了分析。结果表明，施加电压对选择性有微小但有用的影响，可用于微调特定的分离。
