Chan G, Boyle J O, Yang E K, Zhang F, Sacks P G, Shah J P, Edelstein D, Soslow R A, Koki A T, Woerner B M, Masferrer J L, Dannenberg A J
Department of Medicine, New York Presbyterian Hospital and Weill Medical College of Cornell University, New York, NY 10021, USA.
Cancer Res. 1999 Mar 1;59(5):991-4.
The purpose of this study was to determine whether cyclooxygenase-2 (COX-2) was overexpressed in squamous cell carcinoma of the head and neck (HNSCC). Quantitative reverse transcription-PCR, immunoblotting, and immunohistochemistry were used to assess the expression of COX-2 in head and neck tissue. Mean levels of COX-2 mRNA were increased by nearly 150-fold in HNSCC (n = 24) compared with normal oral mucosa from healthy volunteers (n = 17). Additionally, there was about a 50-fold increase in amounts of COX-2 mRNA in normal-appearing epithelium adjacent to HNSCC (n = 10) compared with normal oral mucosa from healthy volunteers. Immunoblotting demonstrated that COX-2 protein was present in six of six cases of HNSCC but was undetectable in normal oral mucosa from healthy subjects. Immunohistochemical analysis showed that COX-2 was expressed in both HNSCC and adjacent normal-appearing epithelium. Taken together, these results suggest that COX-2 may be a target for the prevention or treatment of HNSCC.
本研究的目的是确定环氧化酶-2（COX-2）在头颈部鳞状细胞癌（HNSCC）中是否过表达。采用定量逆转录聚合酶链反应、免疫印迹和免疫组织化学方法评估COX-2在头颈部组织中的表达。与健康志愿者的正常口腔黏膜（n = 17）相比，HNSCC（n = 24）中COX-2 mRNA的平均水平增加了近150倍。此外，与健康志愿者的正常口腔黏膜相比，HNSCC相邻的外观正常上皮（n = 10）中COX-2 mRNA的量增加了约50倍。免疫印迹显示，6例HNSCC中有6例存在COX-2蛋白，但在健康受试者的正常口腔黏膜中未检测到。免疫组织化学分析表明，COX-2在HNSCC和相邻的外观正常上皮中均有表达。综上所述，这些结果表明COX-2可能是预防或治疗HNSCC的一个靶点。
