导数分光光度法同时测定人血浆中吡罗昔康及其主要代谢物5'-羟基吡罗昔康

Simultaneous determination of piroxicam and its major metabolite 5'-hydroxypiroxicam in human plasma by derivative spectrophotometry.

作者信息

Ródenas V, García M S, Sánchez-Pedreño C, Albero M I

机构信息

Department of Analytical Chemistry, Faculty of Chemistry, University of Murcia, Spain.

出版信息

Analyst. 1998 Aug;123(8):1749-52. doi: 10.1039/a802318b.

DOI:10.1039/a802318b

PMID:10071388

Abstract

A first-derivative spectrophotometric method for the simultaneous determination of piroxicam (PX) and its major metabolite 5'-hydroxypiroxicam (OH PX) in human plasma is described. The method consists of direct extraction of the two drugs from the plasma samples with hydrochloric and trichloroacetic acid prior to their determination by measuring the first-derivative signals at 337.0 nm for PX and at 327.0 nm for OHPX (zero-crossing wavelength). The calibration graphs were linear up to 10.0 and 8.0 mg l-1 of PX and OH PX, respectively, and the limits of quantification were 0.27 and 0.56 mg l-1. The possible interfering effects of other substances were studied.

摘要

本文描述了一种用于同时测定人血浆中吡罗昔康（PX）及其主要代谢物5'-羟基吡罗昔康（OH PX）的一阶导数分光光度法。该方法包括先用盐酸和三氯乙酸从血浆样品中直接提取这两种药物，然后通过测量PX在337.0 nm处和OH PX在327.0 nm处（零交叉波长）的一阶导数信号来进行测定。校准曲线分别在PX浓度高达10.0 mg l-1和OH PX浓度高达8.0 mg l-1时呈线性，定量限分别为0.27 mg l-1和0.56 mg l-1。研究了其他物质可能的干扰作用。