Gordillo G, Torres J, Solorzano F, Cedillo-Rivera R, Tapia-Conyer R, Muñoz O
Servicio de Infectologia, Hospital de Pediatría, Centro Médico Nacional Siglo XXI, IMSS, México, D.F.
Arch Med Res. 1999 Jan-Feb;30(1):64-8. doi: 10.1016/s0188-0128(98)00015-3.
Lyme disease is the most common vector-borne human disease in Europe and the United States. In Mexico, clinical cases suggestive of Lyme borreliosis have been reported; however, infection was not confirmed by serologic or microbiologic tests.
To study the prevalence of IgG antibodies against Borrelia burgdorferi among Mexican persons, a community-based sero-survey including all states of Mexico was done. A sample of 2,890 sera representing individuals of all ages and all socioeconomic levels was studied. Antibodies anti-B. burgdorferi were determined by enzyme-linked immunosorbent assay (ELISA) using a whole-cell sonicated extract of B. burgdorferi strain B31. Serum specimens positive for ELISA were further studied by Western blot (WB). A serum sample was considered positive by WB if at least three of the following protein bands were recognized: 18, 24, 28, 29, 31, 34, 39, 41, 45, 58, 62, 66, and 93 kDa. Some WB positive specimens were further confirmed with an immunodot-blot (IDB) test using recombinant and purified B. burgdorferi proteins.
Of the 2,890 specimens, 34 were positive for ELISA; nine of these 34 were confirmed as positive by WB. Four of the nine WB positive sera were tested by IDB and all four were positive. The prevalence of WB confirmed cases in the sample studied was 0.3%. Positive specimens were from residents of the northeastern and central areas of Mexico.
The serological evidences of this study suggest that Borrelia burgdorferi infection is present in the Mexican population. This finding should be confirmed by documenting the infection in clinical cases and in tick vectors.
莱姆病是欧美最常见的媒介传播的人类疾病。在墨西哥，已有提示莱姆病螺旋体病的临床病例报道；然而，感染未通过血清学或微生物学检测得到证实。
为研究墨西哥人群中抗伯氏疏螺旋体IgG抗体的流行情况，开展了一项涵盖墨西哥所有州的基于社区的血清学调查。研究了2890份血清样本，这些样本代表了所有年龄和所有社会经济水平的个体。采用伯氏疏螺旋体菌株B31的全细胞超声裂解提取物，通过酶联免疫吸附测定（ELISA）检测抗伯氏疏螺旋体抗体。ELISA检测呈阳性的血清标本进一步通过免疫印迹法（WB）进行研究。如果免疫印迹法至少识别出以下蛋白条带中的三条，则血清样本被认为呈阳性：18、24、28、29、31、34、39、41、45、58、62、66和93 kDa。部分WB阳性标本进一步通过使用重组和纯化的伯氏疏螺旋体蛋白的免疫斑点印迹（IDB）试验进行确认。
在2890份标本中，34份ELISA检测呈阳性；这34份中的9份经WB确认为阳性。9份WB阳性血清中的4份通过IDB检测，全部4份均为阳性。在所研究的样本中，WB确诊病例的患病率为0.3%。阳性标本来自墨西哥东北部和中部地区的居民。
本研究的血清学证据表明墨西哥人群中存在伯氏疏螺旋体感染。这一发现应通过在临床病例和蜱虫媒介中记录感染情况来加以证实。
