利用铁蛋白抗体复合物对人红细胞H抗原位点进行的电子显微镜观察。

Electron microscopic observations on the H antigen sites of human erythrocytes using ferritin antibody conjugates.

作者信息

Matsukura Y

出版信息

Vox Sang. 1976 Nov;31(5):321-31. doi: 10.1111/j.1423-0410.1976.tb04655.x.

DOI:10.1111/j.1423-0410.1976.tb04655.x

Abstract

Using immunoelectron microscopy, the distribution of the H antigen sites on human erythrocytes was observed in 40 samples of adult, newborn and fetal blood of different ABO phenotypes. The attached ferritin particles indicating the H antigen sites conspicuously varied in number from cell to cell in every specimen. The number of H antigen sites per single red cell was estimated on an average for each sample as follows: O, 3 X 10(5); B, 2 X 10(5); A1, 1.5 X 10(5); A1B, 10(5); A2B, 1.5 X 10(5); Ax, 2.5 X 10(5); AxB, 10(5); Bm, 4 X 10(5); Bw(leukemia), 4 X 10(5); O(newborn), 2.5 X 10(5); B(newborn), 3 X 10(5); A1(newborn), 1.5 X 10(5); A1B(newborn), 2 X 10(5); A1B(fetus), 10(5). The cells in each sample were divided into six cell-populatons according to the number of H antigen sites present. The ratios of distribution of such cell populations are compared for all samples.

摘要

采用免疫电子显微镜技术，对40份不同ABO血型的成人、新生儿和胎儿血液样本中人类红细胞上H抗原位点的分布进行了观察。在每个样本中，指示H抗原位点的附着铁蛋白颗粒数量在细胞之间明显不同。每个样本中单个红细胞上H抗原位点的数量平均估计如下：O型，3×10⁵；B型，2×10⁵；A1型，1.5×10⁵；A1B型，10⁵；A2B型，1.5×10⁵；Ax型，2.5×10⁵；AxB型，10⁵；Bm型，4×10⁵；Bw（白血病），4×10⁵；O型（新生儿），2.5×10⁵；B型（新生儿），3×10⁵；A1型（新生儿），1.5×10⁵；A1B型（新生儿），2×10⁵；A1B型（胎儿），10⁵。根据存在的H抗原位点数量，将每个样本中的细胞分为六个细胞群体。比较了所有样本中这些细胞群体的分布比例。