Huh J, Cho K, Heo D S, Kim J E, Kim C W
Department of Pathology, Asan Medical Center, Ulsan University College of Medicine, Seoul, Korea.
Am J Hematol. 1999 Mar;60(3):205-14. doi: 10.1002/(sici)1096-8652(199903)60:3<205::aid-ajh7>3.0.co;2-j.
One hundred thirty-seven patients with peripheral T-cell lymphomas (PTL) were examined for the presence of Epstein-Barr virus (EBV) using in situ hybridization for EBV-encoded RNA (EBER) and Bam H-fragment, lower strand frame (BHLF) and immunohistochemical stain for latent membrane protein (LMP). EBER was detected in tumor cells in 79 cases (58%); 26/66 PTL, unspecified (39%), 3/4 AILD (75%), 47/51 angiocentric lymphomas (AL) (92%), and 3/13 anaplastic large cell lymphoma (ALCL) (23%) by Revised European-American Lymphoma (REAL) classification. EBER was detected in 17/36 nodal (47%) vs. 62/101 extranodal PTLs (61%); 21/24 nasal, 12/32 Waldeyer's ring, 9/13 gastrointestinal, and 20/32 skin and soft tissue PTL. AL was consistently associated with the highest frequency of EBER among the extranodal PTL: nose (19/20), GI tracts (3/3), skin (14/15), and Waldeyer's ring (11/14). In extranodal lymphomas, coagulative-type zonal necrosis was seen almost exclusively in AL and showed correlation with EBER-positivity (P < 0.01). LMP was detected in 24 among 107 cases tested (22%). No signal for BHLF was detected in 76 cases tested, implying absent or negligible incidence of lytic infection. In conclusion, high incidence of EBV was observed in PTL among Koreans, with predilection for angiocentric lymphomas and extranodal presentation, especially involving nose, skin, and gastrointestinal tract.
对137例外周T细胞淋巴瘤（PTL）患者，采用原位杂交检测EB病毒（EBV）编码的RNA（EBER）和Bam H片段、下链框（BHLF），并对潜伏膜蛋白（LMP）进行免疫组化染色，以检测EBV的存在情况。79例（58%）肿瘤细胞中检测到EBER；根据欧美淋巴瘤修订版（REAL）分类，26/66例未特定的PTL（39%）、3/4例血管免疫母细胞性淋巴结病（AILD）（75%）、47/51例血管中心性淋巴瘤（AL）（92%）以及3/13例间变性大细胞淋巴瘤（ALCL）（23%）检测到EBER。在36例淋巴结PTL中的17例（47%）检测到EBER，而在101例结外PTL中的62例（61%）检测到EBER；21/24例鼻腔、12/32例Waldeyer环、9/13例胃肠道以及20/32例皮肤和软组织PTL。在结外PTL中，AL始终与EBER的最高检出频率相关：鼻腔（19/20）、胃肠道（3/3）、皮肤（14/15）和Waldeyer环（11/14）。在结外淋巴瘤中，凝固型带状坏死几乎仅见于AL，且与EBER阳性相关（P<0.01）。在107例检测病例中的24例（22%）检测到LMP。在76例检测病例中未检测到BHLF信号，这意味着裂解感染的发生率很低或可忽略不计。总之，在韩国人的PTL中观察到EBV的高发生率，血管中心性淋巴瘤和结外表现，尤其是累及鼻腔、皮肤和胃肠道的情况更为常见。
