Harper S J, Buchman V L, Owen D
Department of Anatomy, St. George's Hospital Medical School, Cranmer Terrace, London, Tooting, SW19 0RE, United Kingdom.
Exp Neurol. 1999 Feb;155(2):327-30. doi: 10.1006/exnr.1998.7000.
Following inferior alveolar nerve section and capping of the nerve to prevent regeneration, amounts of nerve growth factor (NGF) and NGF mRNA have been quantified in the chin skin, a discrete target of the nerve. NGF protein in the target region increases rapidly following nerve section and to levels known to induce sprouting. NGF on the ipsilateral side increased many-fold above unoperated control and the contralateral side also increased above control. Measurement of NGF mRNA levels using quantitative Northern analysis revealed, however, that there was no change in the expression levels of NGF mRNA, indicating that the large increase in protein occurs due to a cessation of transport back to the cell bodies of the innervating neurons.
在下牙槽神经切断并对神经进行封端以防止再生后，已对颏部皮肤（该神经的一个离散靶区）中的神经生长因子（NGF）量和NGF mRNA进行了定量。神经切断后，靶区中的NGF蛋白迅速增加，并达到已知可诱导芽生的水平。同侧的NGF增加至未手术对照的许多倍，对侧的NGF也高于对照。然而，使用定量Northern分析测量NGF mRNA水平发现，NGF mRNA的表达水平没有变化，这表明蛋白质的大量增加是由于向支配神经元细胞体的逆向运输停止所致。
