Dechend F, Schubert S, Nanda I, Vogel T, Schmid M, Schmidtke J
Institut für Humangenetik, Medizinische Hochschule, Hannover, Germany.
Cytogenet Cell Genet. 1998;83(3-4):270-4. doi: 10.1159/000015169.
We have isolated both a full-length rat Tspy cDNA from testicular mRNA by rapid amplification of cDNA ends (RACE) and RT-PCR and a full-length rat Tspy gene from genomic DNA by PCR. In contrast to the mouse, where Tspy is present in a single copy and is apparently functionless, and to man and cattle, where TSPY is organized in a moderately repetitive cluster, the rat Tspy locus apparently consists of one complete functional and one truncated, probably nonfunctional, copy, coherently localized on Yp, as revealed by FISH analysis.
我们通过cDNA末端快速扩增法（RACE）和逆转录聚合酶链反应（RT-PCR）从睾丸mRNA中分离出了全长大鼠Tspy cDNA，并通过聚合酶链反应（PCR）从基因组DNA中分离出了全长大鼠Tspy基因。与小鼠（Tspy以单拷贝形式存在且显然无功能）以及人类和牛（TSPY以中度重复簇的形式组织）不同，荧光原位杂交（FISH）分析显示，大鼠Tspy基因座显然由一个完整的功能拷贝和一个截短的、可能无功能的拷贝组成，它们共同定位于Yp。
