Vogel T, Dechend F, Manz E, Jung C, Jakubiczka S, Fehr S, Schmidtke J, Schnieders F
Institut für Humangenetik, Medizinische Hochschule Hannover, D-30623 Hannover, Germany.
Mamm Genome. 1997 Jul;8(7):491-6. doi: 10.1007/s003359900482.
We have isolated genomic sequences as well as transcripts from the bovine homolog of the human testis-specific protein, Y-encoded, TSPY which-in both species-is located on the Y Chromosome (Chr), organized as a gene family with a variable number of members, and expressed exclusively in the testis. 1266 bp of bovine TSPY specific sequence have been isolated from a testis cDNA library, by RT-PCR analyses and by Rapid Amplification of cDNA Ends (RACE). A bovine TSPY gene 4 is organized in seven exons, and transcripts are polyadenylated at various 3' ends. Consensus polyadenylation signals AAUUAAA are missing. Microheterogeneous sequence variation is found between TSPY family members. In addition, homologies to other Y-located repeated sequence families, BRY, have been discovered; these sequences are presumably derived from ancient members of the TSPY cluster, now forming a separate, probably nonfunctional subfamily. Bovine TSPY is subject to differential splicing. In the adult, it is expressed in early germ-cell stages, and expression could also be detected in fetal testis. Comparison with the human homolog shows the highest degree of similarity in the coding regions of exons 2, 3, and 4, which are also precisely conserved regarding their length.
我们已经从人类睾丸特异性蛋白Y编码（TSPY）的牛同源物中分离出基因组序列和转录本，该蛋白在两个物种中均位于Y染色体（Chr）上，组织为一个成员数量可变的基因家族，并且仅在睾丸中表达。通过RT-PCR分析和cDNA末端快速扩增（RACE），从睾丸cDNA文库中分离出了1266 bp的牛TSPY特异性序列。一个牛TSPY基因4由七个外显子组成，转录本在不同的3'末端进行多聚腺苷酸化。共有的多聚腺苷酸化信号AAUUAAA缺失。在TSPY家族成员之间发现了微异质性序列变异。此外，还发现了与其他位于Y染色体上的重复序列家族BRY的同源性；这些序列可能源自TSPY簇的古老成员，现在形成了一个单独的、可能无功能的亚家族。牛TSPY存在差异剪接。在成年个体中，它在早期生殖细胞阶段表达，在胎儿睾丸中也能检测到表达。与人类同源物的比较表明，外显子2、3和4的编码区域相似度最高，并且它们的长度也精确保守。
