Galletta B J, Niu X P, Erickson M R, Abmayr S M
Department of Biochemistry and Molecular Biology, The Pennsylvania State University, University Park, PA 16802, USA.
Gene. 1999 Mar 4;228(1-2):243-52. doi: 10.1016/s0378-1119(99)00010-4.
The vertebrate adapter protein termed Crk was initially identified from the chicken CT10 retrovirus on the basis of its transforming activity (Mayer et al., 1988. Nature 332, 272-275). We have identified a Drosophila protein with homology to vertebrate Crk, termed dCRK, by interaction with the protein encoded by the Drosophila myoblast city (mbc) gene. The dCRK protein has extensive homology to the both the Crk-II form of vertebrate Crk and the Crk-related protein CRKL, and includes one SH2 domain followed by two SH3 domains. A single protein of approx. 37kDa is detected in extracts from embryos, and Northern analysis revealed a single transcript of 1.3kb. The dCrk mRNA is abundant throughout embryogenesis, declines during the larval stages, and reappears during pupation. In addition, maternally-provided transcripts have been detected. During embryogenesis, the spatial distribution of this transcript is relatively broad and appears to include all germ layers. Finally, dCrk is located on the fourth chromosome, approximately at cytological position 101F-102A.
脊椎动物衔接蛋白Crk最初是基于其转化活性从鸡CT10逆转录病毒中鉴定出来的（Mayer等人，1988年。《自然》332卷，272 - 275页）。我们通过与果蝇成肌细胞城市（mbc）基因编码的蛋白质相互作用，鉴定出一种与脊椎动物Crk具有同源性的果蝇蛋白，称为dCRK。dCRK蛋白与脊椎动物Crk的Crk-II形式以及Crk相关蛋白CRKL都具有广泛的同源性，并且包含一个SH2结构域，后面跟着两个SH3结构域。在胚胎提取物中检测到一种约37kDa的单一蛋白质，Northern分析显示有一个1.3kb的单一转录本。dCrk mRNA在整个胚胎发育过程中都很丰富，在幼虫阶段减少，并在化蛹期间重新出现。此外，还检测到了母源提供的转录本。在胚胎发育过程中，该转录本的空间分布相对较广，似乎包括所有胚层。最后，dCrk位于第四条染色体上，大约在细胞学位置101F - 102A处。
