依布硒啉对培养的大脑皮质神经元缺氧损伤的保护作用。
Protection of ebselen against anoxic damage of cultured neurons of cerebral cortex.
作者信息
Tan Y X, Li W H, Tao X B, Jiang Y Y, Chen W P, Zhou B
机构信息
College of Pharmacy, Second Military Medical University, Shanghai, China.
出版信息
Zhongguo Yao Li Xue Bao. 1997 May;18(3):201-3.
AIM
To study the protective effect of ebselen on anoxic damage of brain cells.
METHODS
On d 10 after plating of the cortical neurons from 1-d-old rat, cultures were placed under 95% N2 + 5% CO2 for 2-6 h. Lactate dehydrogenase (LDH) in supernatant, thiobarbituric acid reactive substance (TBARS) and glutathione peroxidase (GSH-Px) activity of neurons were determined.
RESULTS
Under anoxia, efflux of LDH and TBARS from cultured neurons increased while GSH-Px activity decreased. Ebselen reduced the efflux of LDH and TBARS in a dose-related manner and increased the total GSH-Px activity.
CONCLUSION
Ebselen can protect neurons from anoxic damage.
目的
研究依布硒啉对脑细胞缺氧损伤的保护作用。
方法
将1日龄大鼠的皮质神经元接种培养10天后，将培养物置于95% N₂ + 5% CO₂环境中2 - 6小时。测定上清液中的乳酸脱氢酶（LDH）、神经元的硫代巴比妥酸反应物质（TBARS）和谷胱甘肽过氧化物酶（GSH-Px）活性。
结果
缺氧时，培养神经元中LDH和TBARS的流出增加，而GSH-Px活性降低。依布硒啉以剂量相关的方式减少了LDH和TBARS的流出，并增加了总GSH-Px活性。
结论
依布硒啉可保护神经元免受缺氧损伤。
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