人参皂苷对培养的海马神经元缺氧损伤的影响。
Effect of ginsenosides against anoxic damage of hippocampal neurons in culture.
作者信息
Wang F Z, Ding A S, Liu Z W
机构信息
Department of Neurobiology, Academy of Military Medical Sciences, Beijing, China.
出版信息
Zhongguo Yao Li Xue Bao. 1995 Sep;16(5):419-22.
AIM
To study the protective effect of ginsenosides (Gin) against anoxic injury in brain cells.
METHODS
On d 12 after plating of the hippocampal cells from newborn rat, the cultures were exposed to anoxic atmosphere (95% N2 + 5% CO2) for 4-24 h. The cellular morphology, survival rate, and effluxes of lactate dehydrogenase (LDH) and K+ from cells were observed.
RESULTS
After 24-h anoxia, the survival rate of cells was decreased from 92% +/- 4% before anoxia to 1.0% +/- 2.0%; the effluxes of LDH and K+ were increased from 2.3 +/- 0.6 U L-1 and 5.56 +/- 0.16 mmol L-1 before anoxia to 36 +/- 5 U L-1 and 8.5 +/- 0.7 mmol L-1, respectively. In the Gin group exposed anoxia for 24 h, the survival rate of cells was 4% +/- 4%; the effluxes of LDH and K+ were 30 +/- 3 U L-1 and 7.9 +/- 0.8 mmol L-1, respectively. All these changes were lower in Gin group than those of control.
CONCLUSION
Under anoxia the cultured hippocampal neurons were seriously damaged, which may be protected by Gin.
目的
研究人参皂苷（Gin）对脑细胞缺氧损伤的保护作用。
方法
新生大鼠海马细胞接种后第12天，将培养物置于缺氧环境（95% N₂ + 5% CO₂）中4 - 24小时。观察细胞形态、存活率以及细胞中乳酸脱氢酶（LDH）和钾离子（K⁺）的外流量。
结果
缺氧24小时后，细胞存活率从缺氧前的92% ± 4%降至1.0% ± 2.0%；LDH和K⁺的外流量分别从缺氧前的2.3 ± 0.6 U L⁻¹和5.56 ± 0.16 mmol L⁻¹增至36 ± 5 U L⁻¹和8.5 ± 0.7 mmol L⁻¹。在缺氧24小时的Gin组中，细胞存活率为4% ± 4%；LDH和K⁺的外流量分别为30 ± 3 U L⁻¹和7.9 ± 0.8 mmol L⁻¹。Gin组的所有这些变化均低于对照组。
结论
缺氧条件下培养的海马神经元受到严重损伤，Gin可能对其具有保护作用。
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