Wu J F, Zhang J T
Department of Pharmacology, Chinese Academy of Medical Sciences, Beijing, China.
Zhongguo Yao Li Xue Bao. 1999 Jan;20(1):47-51.
To observe the effects of nerve growth factor (NGF) on neuronal hypoxic injury induced by sodium dithionite in primary cultures from gestation of 17-d rat fetal cerebral cortex.
Neuronal death and lactate dehydrogenase (LDH) efflux in the bathing medium were measured. The homogenate of cortical cells was used to determine malonyldialdehyde (MDA) content and superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) activities.
When cultures were incubated for 24 h with sodium dithionite, efflux of LDH and MDA content increased while the number of surviving neurons decreased. NGF 100 micrograms.L-1 increased the number of surviving neurons to 85% +/- 9% of normoxia level under hypoxia. NGF 1-100 micrograms.L-1 concentration-dependently attenuated hypoxia-induced increase of efflux of LDH and MDA content, with IC50 of 27 and 49 (95% confidence limits: 18-49 and 29-110) micrograms.L-1. NGF 30 micrograms.L-1 induced a 3-fold increase in SOD and GSH-Px activities. The levels of SOD and GSH-Px activities in hypoxia group were increased 2.7-fold by NGF 100 micrograms.L-1.
NGF prevented hypoxic insults in cultured cerebral cortical neurons by suppressing the generation of lipid peroxides and increasing the activities of antioxidant enzymes.
观察神经生长因子（NGF）对孕17天大鼠胎脑皮质原代培养物中连二亚硫酸钠诱导的神经元缺氧损伤的影响。
测定神经元死亡情况及培养基中乳酸脱氢酶（LDH）的释放量。用皮质细胞匀浆测定丙二醛（MDA）含量以及超氧化物歧化酶（SOD）和谷胱甘肽过氧化物酶（GSH-Px）的活性。
当培养物用连二亚硫酸钠孵育24小时时，LDH释放量和MDA含量增加，而存活神经元数量减少。100微克.L-1的NGF可使缺氧状态下存活神经元数量增加至常氧水平的85%±9%。1-100微克.L-1的NGF浓度依赖性地减轻缺氧诱导的LDH释放量增加和MDA含量增加，IC50分别为27和49（95%置信限：18-49和29-110）微克.L-1。30微克.L-1的NGF可使SOD和GSH-Px活性增加3倍。100微克.L-1的NGF使缺氧组的SOD和GSH-Px活性水平增加2.7倍。
NGF通过抑制脂质过氧化物的生成并增加抗氧化酶的活性来预防培养的大脑皮质神经元的缺氧损伤。
