三七皂苷对突触体45Ca摄取的影响。
Effect of saponins of Panax notoginseng on synaptosomal 45Ca uptake.
作者信息
Ma L Y, Xiao P G, Liang F Q, Chi M G, Dong S J
机构信息
Institute of Medicinal Plant Development, Chinese Academy of Medical Science, Peking Union Medical College, Beijing, China.
出版信息
Zhongguo Yao Li Xue Bao. 1997 May;18(3):213-5.
AIM
To explore the calcium uptake antagonism of saponins of Panax notoginseng (PNS).
METHODS
Synaptosomes were prepared from rat cerebral cortex by using differential Ficoll gradients. The effects of PNS on synaptosomal 45Ca uptake were measured in vitro or after acute treatment.
RESULTS
PNS 50-800 mg.L-1 produced a concentration-rated inhibition of Ca2+ uptake [IC50 = 111 (46-176) mg.L-1]. Both initial and maximal uptake were inhibited. Similar effect was obtained after acute PNS treatment with 200 mg.kg-1 i.p. The blocking effect of PNS was reversed by calcium in media.
CONCLUSION
PNS is a calcium channel blocker in neurons.
目的
探讨三七总皂苷（PNS）对钙摄取的拮抗作用。
方法
采用差速Ficoll梯度法从大鼠大脑皮层制备突触体。在体外或急性处理后测量PNS对突触体45Ca摄取的影响。
结果
PNS 50 - 800 mg.L-1对Ca2+摄取产生浓度依赖性抑制[IC50 = 111（46 - 176）mg.L-1]。初始摄取和最大摄取均受到抑制。腹腔注射200 mg.kg-1的PNS进行急性处理后也获得了类似的效果。培养基中的钙可逆转PNS的阻断作用。
结论
PNS是神经元中的钙通道阻滞剂。
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