[三七总皂苷对血管紧张素II体外诱导大鼠心肌细胞凋亡的影响]
[Effects of Panax notoginseng saponins on rat cardiomyocytes apoptosis induced by angiotengin II in vitro].
作者信息
Chen Yan-jing, Li Jian-dong, Huang Qi-fu
机构信息
Beijing University of Traditional Chinese Medicine, Beijing 100029, China.
出版信息
Zhongguo Zhong Yao Za Zhi. 2005 May;30(10):778-81.
OBJECTIVE
To study the protective effects of Panax notoginseng saponins (PNS) on angiotensin II (Ang II)-induced rat cardiomyocyte apoptosis in vitro and the probable mechanism.
METHOD
Cultured cardiomyocytes from neonatal rats were stimulated with Ang II. Cell viability was measured by MTT. Apoptosis was evaluated using Acridine Orange (AO) fluorescent dye staining and flow cytometry; Fluo-3 AM was used to test the change of intracellular free calcium.
RESULT
It was found that incubating with Ang II (10(-7) mol x L(-1)) for 48 h increased cardiomyocyte apoptosis, PNS (25, 100 mg x mL(-1)) increased myocyte viability. PNS (50 mg x mL(-1)) significantly decreased this Ang II-induced rat cardiomyocyte apoptosis (P < 0.05) and decreased fluorescent intensity of intracellular calcium.
CONCLUSION
PNS has a significant effect on Ang II-induced rat cardiomyocytes apoptosis in vitro by alleviating intracellular calcium overload.
目的
研究三七总皂苷（PNS）对血管紧张素II（Ang II）诱导的大鼠心肌细胞凋亡的体外保护作用及其可能机制。
方法
用Ang II刺激新生大鼠培养的心肌细胞。采用MTT法检测细胞活力。用吖啶橙（AO）荧光染料染色和流式细胞术评估细胞凋亡；用Fluo-3 AM检测细胞内游离钙的变化。
结果
发现用Ang II（10(-7) mol·L(-1)）孵育48小时可增加心肌细胞凋亡，PNS（25、100 mg·mL(-1)）可提高心肌细胞活力。PNS（50 mg·mL(-1)）可显著降低Ang II诱导的大鼠心肌细胞凋亡（P < 0.05），并降低细胞内钙的荧光强度。
结论
PNS通过减轻细胞内钙超载对Ang II诱导的大鼠心肌细胞凋亡具有显著作用。
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