Zhang X W, Niu X L, Guo Z G
Laboratory of Molecular Pharmacology, Hunan Medical University, Changsha, China.
Zhongguo Yao Li Xue Bao. 1997 May;18(3):267-70.
To study whether estrogen might induce apoptosis in mouse peritoneal macrophages (MPM).
The MPM were isolated and incubated in culture medium containing 17-beta-estradiol, estrone, or equal volume of 100% ethanol as control. DNA fragmentation was visualized by agarose gel electrophoresis.
17-beta-Estradiol 0.01-1 mumol.L-1 or estrone 10-20 mumol.L-1 elicited typical morphological apoptosis and DNA fragmentation in a concentration-dependent manner in MPM. Staurosporine (Sta) 0.01 mumol.L-1, cycloheximide (Cyc) 1 mg.L-1, and tamoxifen (Tam) 10 mumol.L-1 inhibited the DNA fragmentation induced by 17-beta-estradiol 1 mumol.L-1 or estrone 20 mumol.L-1.
Estradiol and estrone induced apoptosis in MPM.
研究雌激素是否可诱导小鼠腹腔巨噬细胞（MPM）凋亡。
分离MPM并在含有17-β-雌二醇、雌酮或等体积100%乙醇作为对照的培养基中培养。通过琼脂糖凝胶电泳观察DNA片段化。
0.01 - 1 μmol·L⁻¹的17-β-雌二醇或10 - 20 μmol·L⁻¹的雌酮以浓度依赖方式在MPM中引发典型的形态学凋亡和DNA片段化。0.01 μmol·L⁻¹的星形孢菌素（Sta）、1 mg·L⁻¹的环己酰亚胺（Cyc）和10 μmol·L⁻¹的他莫昔芬（Tam）抑制了1 μmol·L⁻¹的17-β-雌二醇或20 μmol·L⁻¹的雌酮诱导的DNA片段化。
雌二醇和雌酮可诱导MPM凋亡。
