从与泰国红树植物木果楝相关的担子菌内生真菌XG8D中分离得到的菖蒲烷倍半萜。

Choodej Siwattra, Teerawatananond Thapong, Mitsunaga Tohru, Pudhom Khanitha

The United Graduate School of Agricultural Science, Gifu University, 1-1 Yanagido, Gifu 501-1193, Japan.

Faculty of Science and Technology, Valaya Alongkorn Rajabhat University under Royal Patronage, Pathumtani 13138, Thailand.

Mar Drugs. 2016 Jul 15;14(7):132. doi: 10.3390/md14070132.

Six new chamigrane sesquiterpenes, merulinols A‒F (1‒6), and four known metabolites (7‒10) were isolated from the culture of the basidiomycetous fungus XG8D, a mangrove-derived endophyte. Their structures were elucidated mainly by 1D and 2D NMR, while the structures of 1 and 2 were further confirmed by single-crystal X-ray diffraction analysis. The in vitro cytotoxicity of all compounds was evaluated against three human cancer cell lines, MCF-7, Hep-G2, and KATO-3. Compounds 3 and 4 selectively displayed cytotoxicity against KATO-3 cells with IC50 values of 35.0 and 25.3 μM, respectively.

从一种源自红树林的内生担子菌XG8D的培养物中分离出了6种新的菖蒲烷倍半萜类化合物，即merulinols A - F（1 - 6），以及4种已知代谢产物（7 - 10）。它们的结构主要通过一维和二维核磁共振进行阐明，而化合物1和2的结构通过单晶X射线衍射分析进一步得到确证。评估了所有化合物对三种人类癌细胞系MCF - 7、Hep - G2和KATO - 3的体外细胞毒性。化合物3和4对KATO - 3细胞选择性地表现出细胞毒性，IC50值分别为35.0和25.3 μM。