Ma T C, Zhu X Z
Department of Pharmacology, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, China.
Zhongguo Yao Li Xue Bao. 1997 Mar;18(2):121-3.
To study the effect of intrahippocampal infusion of interleukin-6 (IL-6) on active avoidance in rats and the possible involvement of nitric oxide (NO).
Using a shuttle-box model, the effects of bilaterally intrahippocampal infusion of IL-6 3.2, 16, and 80 ng as well as sodium nitroprusside (SNP) 400 ng on active avoidance were studied on d 8 after administration. The levels of nitrite as an index of NO in the hippocampus were detected using a fluorometric assay 24 h after infusion of IL-6 3.2 or 80 ng.
IL-6 16 and 80 ng impaired the acquisition performance of active avoidance by prolonging the latency of avoidance in training, but not the retention performance in testing. IL-680 ng and SNP 400 ng also resulted in a marked impairment in acquisition performances by decreasing the rate of avoidance, but not in retention performances. IL-680 ng markedly elevated the nitrite levels from 10.6 +/- 0.7 in control rats to 13.6 +/- 2.0 (nmol/g wet wt) (P < 0.01). IL-6 3.2 ng had no effect on active avoidance nor on nitrite levels.
Intrahippocampal infusion of IL-6 impaired learning acquisition of active avoidance in rats.
研究海马内注射白细胞介素-6（IL-6）对大鼠主动回避行为的影响以及一氧化氮（NO）可能的参与作用。
采用穿梭箱模型，在给药后第8天研究双侧海马内注射3.2、16和80 ng IL-6以及400 ng硝普钠（SNP）对主动回避行为的影响。在注射3.2或80 ng IL-6后24小时，使用荧光测定法检测海马中作为NO指标的亚硝酸盐水平。
16和80 ng IL-6通过延长训练中回避的潜伏期损害了主动回避行为的习得表现，但不影响测试中的记忆保持表现。80 ng IL-6和400 ng SNP也通过降低回避率导致习得表现明显受损，但不影响记忆保持表现。80 ng IL-6使亚硝酸盐水平从对照大鼠的10.6±0.7显著升高至13.6±2.0（nmol/g湿重）（P<0.01）。3.2 ng IL-6对主动回避行为和亚硝酸盐水平均无影响。
海马内注射IL-6损害了大鼠主动回避行为的学习习得。
