Zeng Q, Zhou J, Zeng X, Peng X, Zhang S, Yi X
Department of Parasitology, Hunan Medical University, Changsha.
Hunan Yi Ke Da Xue Xue Bao. 1997;22(6):475-7.
Cross reactions of T. spiralis muscular larva antigens (TsMLA) against several sera from patients with other parasitic diseases by ELIB (enzyme-linked immunoblotting technique) were studied. The proteins of 31-100 KDa were recognized by sera of two cases of acute schistosomiasis, while proteins of 44/45, 51/53, 62/64 and 100 KDa were recognized by sera of 11 cases of chronic schistosomiasis. The proteins of 60 KDa showed varied cross reactions against sera of five kinds of patients with other parasitic diseases (filariasis, hookworm diseases, paragonimiasis, ascariasis and clonorchiasis). Only the proteins of 45 KDa were recognized by six of fifteen cases of normal human sera. All of > 29 KDa proteins were recognized by sera of rabbit infected by T. spiralis. It is suggested that there were many antigens in Trichinellen muscular larva which shared in common with several other parasites. The cross reactions of TsMLA against Schistosoma japonicum may be differentiated on the positive reaction bands of 44/45, 51/53, 62/64 and 100 KDa proteins which was specific to the chronic schistosomiasis using the TsMLA by means of ELIB.
采用酶联免疫印迹技术（ELIB）研究了旋毛虫肌幼虫抗原（TsMLA）与其他寄生虫病患者的几份血清的交叉反应。两例急性血吸虫病患者的血清识别出31 - 100 kDa的蛋白质，而11例慢性血吸虫病患者的血清识别出44/45、51/53、62/64和100 kDa的蛋白质。60 kDa的蛋白质与其他五种寄生虫病（丝虫病、钩虫病、肺吸虫病、蛔虫病和华支睾吸虫病）患者的血清呈现出不同的交叉反应。15例正常人血清中有6例识别出仅45 kDa的蛋白质。所有大于29 kDa的蛋白质均被旋毛虫感染的兔血清识别。提示旋毛虫肌幼虫中存在许多与其他几种寄生虫共有的抗原。利用ELIB技术，通过TsMLA，可根据慢性血吸虫病特有的44/45、51/53、62/64和100 kDa蛋白质的阳性反应条带区分TsMLA与日本血吸虫的交叉反应。
