Linder E, Thors C, Lundin L, Ljungström I, Farah S, Hagi H, Dias F
Department of Parasitology, National Bacteriological Laboratory, Stockholm, Sweden.
J Parasitol. 1992 Dec;78(6):999-1005.
The 50-kDa component (gp50) present in Schistosoma mansoni eggs and secretions of the various life stages of the parasite was recognized by experimentally infected mice and by humans with S. mansoni, Schistosoma haematobium, and Schistosoma japonicum infection. All sera reacting with crude S. mansoni-soluble egg antigens (SEA) also reacted strongly with gp50 in enzyme-linked immunosorbent assay. No reactivity against gp50 was seen with sera from individuals without schistosomiasis, with the exception of sera from patients with Trichinella spiralis infection. All of 10 sera from patients with trichinellosis also reacted with schistosomes by immunofluorescence essentially recognizing testes, ovaries, ootype epithelium and ducts of the reproductive system. Cross-reacting antigens were seen in T. spiralis hypodermis, stichocytes and possibly germinal primordia using anti-gp50 monoclonal antibodies and anti-gp50-positive schistosomiasis patient sera. The results suggest that the anti-gp50 antibody response constitutes a significant part of the anti-SEA antibody response in infected individuals and is a major reason for the previously recognized serological cross-reactivity between T. spiralis and schistosome species.
曼氏血吸虫卵以及该寄生虫各个生活阶段分泌物中存在的50-kDa成分（gp50），能被实验感染的小鼠以及感染曼氏血吸虫、埃及血吸虫和日本血吸虫的人类识别。在酶联免疫吸附测定中，所有与曼氏血吸虫可溶性虫卵粗抗原（SEA）发生反应的血清，也都与gp50发生强烈反应。除旋毛虫感染患者的血清外，未感染血吸虫病个体的血清对gp50无反应性。10例旋毛虫病患者的血清通过免疫荧光法也与血吸虫发生反应，主要识别生殖系统的睾丸、卵巢、卵模上皮和管道。使用抗gp50单克隆抗体和抗gp50阳性血吸虫病患者血清，在旋毛虫的皮下组织、杆状体细胞以及可能的生殖原基中发现了交叉反应抗原。结果表明，抗gp50抗体反应在受感染个体的抗SEA抗体反应中占很大一部分，并且是先前已确认的旋毛虫与血吸虫物种之间血清学交叉反应的主要原因。
