Diwakarla C S, Palombo E A
J Gen Virol. 1999 Feb;80 ( Pt 2):341-344. doi: 10.1099/0022-1317-80-2-341.
The deduced amino acid sequences of the outer capsid protein, VP7, of serotype G1 rotavirus clinical isolates collected over a 6 year period (1990-1995) in Melbourne, Australia, were examined. Phylogenetic analysis characterized the sequences into two discrete clusters representing two of the four global lineages of human G1 VP7 proteins. Antigenic characterization using a panel of serotype G1-specific neutralizing monoclonal antibodies classified lineage II isolates (1990-1993) as monotype G1a while lineage I isolates were classified as monotype G1b (1993-1995). Examination of the sequences of the neutralization epitope regions of VP7 revealed a particular amino acid substitution at residue 94 in region A (Asp --> Ser/Thr) that correlated with lineage and monotype designation. Our results indicated that temporal genetic variation of the VP7 of serotype G1 rotaviruses was associated with changes in the antigenicity of these isolates.
对1990年至1995年期间在澳大利亚墨尔本收集的G1型轮状病毒临床分离株的外衣壳蛋白VP7推导的氨基酸序列进行了研究。系统发育分析将这些序列分为两个离散的簇，代表人类G1型VP7蛋白四个全球谱系中的两个。使用一组G1型特异性中和单克隆抗体进行抗原特性分析，将II型谱系分离株（1990 - 1993年）分类为G1a单型，而I型谱系分离株分类为G1b单型（1993 - 1995年）。对VP7中和表位区域序列的研究揭示了A区域第94位残基处的一个特定氨基酸取代（天冬氨酸→丝氨酸/苏氨酸），该取代与谱系和单型分类相关。我们的结果表明，G1型轮状病毒VP7的时间遗传变异与这些分离株的抗原性变化有关。
