Palombo E A, Bugg H C, Masendycz P J, Bishop R F
Department of Gastroenterology, Royal Children's Hospital, Parkville, Victoria, Australia.
DNA Seq. 1997;7(5):307-11. doi: 10.3109/10425179709034050.
The nucleotide sequence of the gene encoding the outer capsid glycoprotein, VP7, isolated from a reassortant human rotavirus, M3014, was determined. The deduced amino acid sequence exhibited significant identity to the VP7 from a standard strain belonging to serotype G4, although the antigenic regions of the M3014 VP7 resembled sequences from both serotype G4 and G9 viruses. However, reactivity with G4 or G9 serotype-specific monoclonal antibodies was not observed. We suggest that the M3014 VP7 was derived from sequential mutation of a G4-like progenitor gene resulting in a protein with novel antigenic properties.
测定了从重组人轮状病毒M3014中分离出的编码外衣壳糖蛋白VP7的基因的核苷酸序列。推导的氨基酸序列与属于G4血清型的标准毒株的VP7具有显著的同一性，尽管M3014 VP7的抗原区域类似于G4和G9病毒血清型的序列。然而，未观察到与G4或G9血清型特异性单克隆抗体的反应性。我们认为，M3014 VP7源自类似G4的祖先基因的连续突变，产生了具有新抗原特性的蛋白质。
