Coulson B S, Kirkwood C
Department of Gastroenterology, Royal Children's Hospital, Parkville, Victoria, Australia.
J Virol. 1991 Nov;65(11):5968-74. doi: 10.1128/JVI.65.11.5968-5974.1991.
The neutralization epitopes of the VP7 of human rotavirus RV-4 were studied by using five neutralizing mouse monoclonal antibodies to select virus variants resistant to neutralization by each of the antibodies. Antibody resistance patterns and sequence analysis of the RV-4 variants revealed that at least four sites on VP7, located at amino acids 94 (region A), 147 to 148 (region B), 213 (region C), and 291, are involved in neutralization of the human G1 rotavirus RV-4. The A-region site elicited antibody cross-reactive between G types and showed species-restricted immunodominance not related to carbohydrate attachment. The monotype 1b rotavirus M37 lacked this site. The B region contained strain-specific and cross-reactive sites, absent in monotype 1c rotaviruses. The C-region site was present in all G1 rotaviruses tested. Monotype 1a rotaviruses contained all these sites of neutralization. Virus monotype and sensitivity to monoclonal antibody neutralization usually related to the presence of a particular amino acid(s) at or next to the positions at which the mutations were selected in the virus variants.
通过使用五种中和性小鼠单克隆抗体来选择对每种抗体中和作用具有抗性的病毒变体，对人轮状病毒RV-4的VP7中和表位进行了研究。RV-4变体的抗体抗性模式和序列分析表明，VP7上至少有四个位点，分别位于氨基酸94（A区）、147至148（B区）、213（C区）和291，参与了人G1轮状病毒RV-4的中和作用。A区位点引发了不同G型之间的抗体交叉反应，并表现出与碳水化合物附着无关的种属限制性免疫显性。1b型轮状病毒M37缺乏该位点。B区包含1c型轮状病毒中不存在的毒株特异性和交叉反应位点。C区位点存在于所有测试的G1轮状病毒中。1a型轮状病毒包含所有这些中和位点。病毒的型别和对单克隆抗体中和作用的敏感性通常与病毒变体中选择突变的位置处或其附近特定氨基酸的存在有关。
