Hessner M J, Pircon R A, Johnson S T, Luhm R A
The Diagnostic Laboratories of The Blood Center, Milwaukee, WI 53233, USA.
Prenat Diagn. 1999 Jan;19(1):41-5. doi: 10.1002/(sici)1097-0223(199901)19:1<41::aid-pd463>3.0.co;2-a.
Maternal allo-immunization to antigens of the Duffy blood group system can result in haemolytic disease of the newborn (HDN), therefore, the application of allele-specific polymerase chain reaction (ASPCR) for prenatal genotyping of the Duffy antigen system to identify pregnancies at risk for HDN was evaluated. Oligonucleotide primers were designed for ASPCR of FYA, FYB and nullFY alleles. A validation study was performed using DNA isolated from 94 serotyped whole blood samples and 8 amniocentesis samples. A concordance rate of 100 per cent was observed between serotyping and ASPCR detection of the FYA, FYB and nullFY alleles. This assay is particularly useful for rapid genotyping of fetal amniotic cells to identify pregnancies at risk for HDN due to maternal fetal incompatibilities within the Duffy blood group system.
母体对达菲血型系统抗原的同种免疫可导致新生儿溶血病（HDN），因此，评估了应用等位基因特异性聚合酶链反应（ASPCR）对达菲抗原系统进行产前基因分型以识别有HDN风险的妊娠。设计了用于FYA、FYB和nullFY等位基因ASPCR的寡核苷酸引物。使用从94份血清分型全血样本和8份羊水穿刺样本中分离的DNA进行了验证研究。在FYA、FYB和nullFY等位基因的血清分型和ASPCR检测之间观察到100%的一致性率。该检测方法对于快速对胎儿羊水细胞进行基因分型以识别由于达菲血型系统内母婴不相容而有HDN风险的妊娠特别有用。
