IncL/M质粒repA基因假结依赖性表达与调控相关元件的分析
Analysis of elements involved in pseudoknot-dependent expression and regulation of the repA gene of an IncL/M plasmid.
作者信息
Athanasopoulos V, Praszkier J, Pittard A J
机构信息
Department of Microbiology and Immunology, The University of Melbourne, Parkville, Victoria 3052, Australia.
出版信息
J Bacteriol. 1999 Mar;181(6):1811-9. doi: 10.1128/JB.181.6.1811-1819.1999.
Abstract
Replication of the IncL/M plasmid pMU604 is controlled by a small antisense RNA molecule (RNAI), which, by inhibiting the formation of an RNA pseudoknot, regulates translation of the replication initiator protein, RepA. Efficient translation of the repA mRNA was shown to require the translation and correct termination of the leader peptide, RepB, and the formation of the pseudoknot. Although the pseudoknot was essential for the expression of repA, its presence was shown to interfere with the translation of repB. The requirement for pseudoknot formation could in large part be obviated by improving the ribosome binding region of repA, either by replacing the GUG start codon by AUG or by increasing the spacing between the start codon and the Shine-Dalgarno sequence (SD). The spacing between the distal pseudoknot sequence and the repA SD was shown to be suboptimal for maximal expression of repA.
摘要
IncL/M 质粒 pMU604 的复制受一个小反义 RNA 分子（RNAI）控制，该分子通过抑制 RNA 假结的形成来调节复制起始蛋白 RepA 的翻译。研究表明，repA mRNA 的有效翻译需要前导肽 RepB 的翻译和正确终止以及假结的形成。虽然假结对 repA 的表达至关重要，但它的存在会干扰 repB 的翻译。通过将 repA 的 GUG 起始密码子替换为 AUG 或增加起始密码子与 Shine-Dalgarno 序列（SD）之间的间距来改善 repA 的核糖体结合区域，在很大程度上可以避免对假结形成的需求。结果表明，远端假结序列与 repA SD 之间的间距对于 repA 的最大表达并非最优。
相似文献
1
Analysis of elements involved in pseudoknot-dependent expression and regulation of the repA gene of an IncL/M plasmid.IncL/M质粒repA基因假结依赖性表达与调控相关元件的分析
J Bacteriol. 1999 Mar;181(6):1811-9. doi: 10.1128/JB.181.6.1811-1819.1999.
2
The replication of an IncL/M plasmid is subject to antisense control.IncL/M 质粒的复制受反义控制。
J Bacteriol. 1995 Aug;177(16):4730-41. doi: 10.1128/jb.177.16.4730-4741.1995.
3
Pseudoknot-dependent translational coupling in repBA genes of the IncB plasmid pMU720 involves reinitiation.IncB质粒pMU720的repBA基因中依赖假结的翻译偶联涉及重新起始。
J Bacteriol. 2002 Oct;184(20):5772-80. doi: 10.1128/JB.184.20.5772-5780.2002.
4
Replication control of plasmid R1: disruption of an inhibitory RNA structure that sequesters the repA ribosome-binding site permits tap-independent RepA synthesis.质粒R1的复制控制：隔离repA核糖体结合位点的抑制性RNA结构的破坏允许不依赖tap的RepA合成。
Mol Microbiol. 1994 Apr;12(1):49-60. doi: 10.1111/j.1365-2958.1994.tb00994.x.
5
Importance of structural differences between complementary RNA molecules to control of replication of an IncB plasmid.互补RNA分子之间的结构差异对IncB质粒复制控制的重要性。
J Bacteriol. 1997 Feb;179(3):742-53. doi: 10.1128/jb.179.3.742-753.1997.
6
Mutations affecting pseudoknot control of the replication of B group plasmids.影响B组质粒复制假结控制的突变。
J Bacteriol. 1993 Oct;175(20):6476-83. doi: 10.1128/jb.175.20.6476-6483.1993.
7
Control of replication in I-complex plasmids.I 型复合质粒复制的控制
Plasmid. 2005 Mar;53(2):97-112. doi: 10.1016/j.plasmid.2004.12.005.
8
The replicon of pSW800 from Pantoea stewartii.来自斯氏泛菌的pSW800复制子。
Microbiology (Reading). 2001 Oct;147(Pt 10):2757-2767. doi: 10.1099/00221287-147-10-2757.
9
Mutations affecting translational coupling between the rep genes of an IncB miniplasmid.影响IncB小质粒rep基因间翻译偶联的突变
J Bacteriol. 1992 Apr;174(7):2376-83. doi: 10.1128/jb.174.7.2376-2383.1992.
10
Molecular analysis of RNAI control of repB translation in IncB plasmids.IncB质粒中repB翻译的RNA干扰控制的分子分析。
J Bacteriol. 1994 Nov;176(21):6497-508. doi: 10.1128/jb.176.21.6497-6508.1994.
引用本文的文献
1
Genetic determinants of pOXA-48 plasmid maintenance and propagation in Escherichia coli.大肠杆菌中pOXA - 48质粒维持与传播的遗传决定因素
Nat Commun. 2025 Aug 19;16(1):7734. doi: 10.1038/s41467-025-62404-7.
2
Understanding the genetic basis of the incompatibility of IncK1 and IncK2 plasmids.了解IncK1和IncK2质粒不相容性的遗传基础。
Open Res Eur. 2023 Oct 30;3:53. doi: 10.12688/openreseurope.15121.2. eCollection 2023.
3
LaRA 2: parallel and vectorized program for sequence-structure alignment of RNA sequences.LaRA 2：用于 RNA 序列序列-结构比对的并行和矢量化程序。
BMC Bioinformatics. 2022 Jan 6;23(1):18. doi: 10.1186/s12859-021-04532-7.
4
pCTX-M3-Structure, Function, and Evolution of a Multi-Resistance Conjugative Plasmid of a Broad Recipient Range.pCTX-M3-结构、功能与广泛宿主范围的多重耐药可移动质粒的演化
Int J Mol Sci. 2021 Apr 27;22(9):4606. doi: 10.3390/ijms22094606.
5
Mechanisms of Theta Plasmid Replication in Enterobacteria and Implications for Adaptation to Its Host.肠杆菌中θ质粒复制的机制及其对宿主适应性的影响
EcoSal Plus. 2020 Nov;9(1). doi: 10.1128/ecosalplus.ESP-0026-2019.
6
OXA-48-Mediated Ceftazidime-Avibactam Resistance Is Associated with Evolutionary Trade-Offs.OXA-48 介导的头孢他啶-阿维巴坦耐药与进化权衡有关。
mSphere. 2019 Mar 27;4(2):e00024-19. doi: 10.1128/mSphere.00024-19.
7
Identification of Plasmid-Encoded sRNAs in a -Harboring Multidrug-Resistance Plasmid pNDM-HK in .在[具体地点]中携带多重耐药质粒pNDM-HK的[细菌名称]中鉴定质粒编码的小RNA
Front Microbiol. 2018 Mar 27;9:532. doi: 10.3389/fmicb.2018.00532. eCollection 2018.
8
Genome-based insights into the resistome and mobilome of multidrug-resistant Aeromonas sp. ARM81 isolated from wastewater.基于基因组对从废水中分离出的多重耐药气单胞菌ARM81的耐药组和可移动遗传元件组的深入了解。
Arch Microbiol. 2017 Jan;199(1):177-183. doi: 10.1007/s00203-016-1285-6. Epub 2016 Sep 2.
9
Why is start codon selection so precise in eukaryotes?为什么起始密码子的选择在真核生物中如此精确？
Translation (Austin). 2014 Mar 12;2(1):e28387. doi: 10.4161/trla.28387. eCollection 2014.
10
Complete nucleotide sequence of the pCTX-M3 plasmid and its involvement in spread of the extended-spectrum beta-lactamase gene blaCTX-M-3.pCTX-M3质粒的完整核苷酸序列及其在超广谱β-内酰胺酶基因blaCTX-M-3传播中的作用。
Antimicrob Agents Chemother. 2007 Nov;51(11):3789-95. doi: 10.1128/AAC.00457-07. Epub 2007 Aug 13.
本文引用的文献
1
[The biosynthesis of beta-galactosidase (lactase) in Escherichia coli; the specificity of induction].[大肠杆菌中β-半乳糖苷酶（乳糖酶）的生物合成；诱导的特异性]
Biochim Biophys Acta. 1951 Nov;7(4):585-99. doi: 10.1016/0006-3002(51)90072-8.
2
Structural basis for binding of the plasmid ColIb-P9 antisense Inc RNA to its target RNA with the 5'-rUUGGCG-3' motif in the loop sequence.质粒ColIb-P9反义Inc RNA与环序列中具有5'-rUUGGCG-3'基序的靶RNA结合的结构基础。
J Biol Chem. 1998 May 8;273(19):11826-38. doi: 10.1074/jbc.273.19.11826.
3
An RNA pseudoknot as the molecular switch for translation of the repZ gene encoding the replication initiator of IncIalpha plasmid ColIb-P9.一种RNA假结作为IncIα质粒ColIb-P9复制起始因子repZ基因翻译的分子开关。
J Biol Chem. 1998 May 8;273(19):11815-25. doi: 10.1074/jbc.273.19.11815.
4
Importance of structural differences between complementary RNA molecules to control of replication of an IncB plasmid.互补RNA分子之间的结构差异对IncB质粒复制控制的重要性。
J Bacteriol. 1997 Feb;179(3):742-53. doi: 10.1128/jb.179.3.742-753.1997.
5
6
A genetic analysis of various functions of the TyrR protein of Escherichia coli.大肠杆菌TyrR蛋白多种功能的遗传分析。
J Bacteriol. 1993 Mar;175(6):1767-76. doi: 10.1128/jb.175.6.1767-1776.1993.
7
Footprinting mRNA-ribosome complexes with chemical probes.用化学探针绘制mRNA-核糖体复合物的足迹图谱。
EMBO J. 1994 Aug 15;13(16):3892-901. doi: 10.1002/j.1460-2075.1994.tb06700.x.
8
How the ribosome moves along the mRNA during protein synthesis.核糖体在蛋白质合成过程中如何沿着信使核糖核酸移动。
J Biol Chem. 1994 Dec 2;269(48):30713-7.
9
Regulation of aroL expression by TyrR protein and Trp repressor in Escherichia coli K-12.大肠杆菌K-12中TyrR蛋白和色氨酸阻遏物对aroL表达的调控。
J Bacteriol. 1994 Nov;176(22):6921-30. doi: 10.1128/jb.176.22.6921-6930.1994.
10
Mutations affecting pseudoknot control of the replication of B group plasmids.影响B组质粒复制假结控制的突变。
J Bacteriol. 1993 Oct;175(20):6476-83. doi: 10.1128/jb.175.20.6476-6483.1993.
Zotero 插件