影响B组质粒复制假结控制的突变。
Mutations affecting pseudoknot control of the replication of B group plasmids.
作者信息
Wilson I W, Praszkier J, Pittard A J
机构信息
Department of Microbiology, University of Melbourne, Parkville, Victoria, Australia.
出版信息
J Bacteriol. 1993 Oct;175(20):6476-83. doi: 10.1128/jb.175.20.6476-6483.1993.
Abstract
The translational initiation region of the mRNA for the replication initiation protein (RepA) of pMU720 is predicted to be sequestered in an inhibitory secondary structure designated stem-loop III. Activation of repA translation requires both the disruption of stem-loop III by ribosomes involved in the translation and termination of the leader peptide RepB and the formation of a pseudoknot, a tertiary RNA structure. Disruption of stem-loop III by site-directed mutagenesis was found to be insufficient to allow high repA expression in the absence of pseudoknot formation, indicating that the pseudoknot acts as an enhancer of repA translation. Furthermore, extending the length of the leader peptide RepB and changing the distance between the pseudoknot and repA Shine-Dalgarno sequence were found to have major effects on the translation of repA.
摘要
pMU720复制起始蛋白（RepA）的mRNA的翻译起始区域预计被隔离在一个名为茎环III的抑制性二级结构中。repA翻译的激活既需要参与翻译的核糖体破坏茎环III以及前导肽RepB的终止，也需要假结（一种三级RNA结构）的形成。通过定点诱变破坏茎环III被发现不足以在没有假结形成的情况下实现高repA表达，这表明假结作为repA翻译的增强子。此外，延长前导肽RepB的长度以及改变假结与repA的Shine-Dalgarno序列之间的距离被发现对repA的翻译有重大影响。
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